SAA1 Antikörper

Produktnummer ABIN305737

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SAA1 in ELISA, WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SAA1 Antikörper (ABIN305737)

Target: SAA1 (Serum Amyloid A1 (SAA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SAA1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SAA1 Antikörper

Marke: IHC-plus™

Spezifität: Recognizes natural and recombinant human SAA. Does not show any cross-reactivity with other human cytokines or growth factors tested such as IL-1b, IL-8, MCAF, TGF-b and EGF.

Aufreinigung: Protein G purified

Immunogen: Highly purified recombinant human SAA, 12kD.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.3 μg/mL), IHC, IHC-P (5 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA (capture coating): 0.3 μg/mL Detects 1.1 ng/mL of SAA in serum/plasma or 0.6 ng/mL of SAA in medium with an assay range of 0 to 80 ng/mL. Suggested substrate: TMB. Western Blot: 0.1-0.5 μg allows visualization of 100 ng/lane of recombinant human SAA. Used in sandwich ELISA as the capture antibody with LS-B2208 as the detection antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SAA1

Target: SAA1 (Serum Amyloid A1 (SAA1))

Andere Bezeichnung: SAA1 / SAA / Serum Amyloid A

Hintergrund: Name/Gene ID: SAA1

Synonyms: SAA1, Serum amyloid A protein, Serum Amyloid A, Serum amyloid A-1 protein, TP53I4, SAA, Serum amyloid A1, PIG4

Gen-ID: 6288

UniProt: P02735

Pathways: Toll-Like Receptors Cascades