TJP1 Antikörper (AA 463-1109)

Produktnummer ABIN305736

Dieses Anti-TJP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TJP1 in WB, IHC, IP, ELISA, IHC (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Hund und Meerschweinchen.

Kurzübersicht für TJP1 Antikörper (AA 463-1109) (ABIN305736)

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-TJP1 Antikörper

Bindungsspezifität: AA 463-1109

Marke: IHC-plus™

Spezifität: Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.

Aufreinigung: Immunoaffinity purified

Immunogen: A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 2-5 μg/reaction.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Andere Bezeichnung: TJP1 / ZO-1

Hintergrund: Name/Gene ID: TJP1
Family: Occludin

Synonyms: TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein

Gen-ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization