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His Tag Antikörper (FITC)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch His Tag in IHC und FACS. Er zeigt eine Reaktivität gegenüber .
Produktnummer ABIN304882

Kurzübersicht für His Tag Antikörper (FITC) (ABIN304882)

Target

Alle His Tag Antikörper anzeigen
His Tag

Reaktivität

Bitte anfragen

Wirt

  • 118
  • 64
  • 11
  • 10
  • 9
  • 8
  • 2
  • 1
Maus

Klonalität

  • 135
  • 74
  • 10
Monoklonal

Konjugat

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  • 1
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  • 1
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Dieser His Tag Antikörper ist konjugiert mit FITC

Applikation

  • 176
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Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon

AD1-1-10
  • Sequenz

    HHHHHH

    Spezifität

    Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

    Aufreinigung

    Protein G purified

    Immunogen

    PAX6 transcription factor linked to histidine tag.

    Type of Immunogen: Fusion protein

    Isotyp

    IgG1
  • Applikationshinweise

    Approved: Flo (1:10), IHC

    Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    PBS, 0.09 % sodium azide, 1 % BSA.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
  • Target

    His Tag

    Substanzklasse

    Tag
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