beta Actin Antikörper (N-Term)

Produktnummer ABIN3044573

Produktdetails anti-beta Actin Antikörper

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Bindungsspezifität: AA 2-19, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Actin, cytoplasmic 1(ACTB) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: DDDIAALVVD NGSGMCKA

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Actin, cytoplasmic 1(ACTB) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: actin, beta
Protein Name: Actin, cytoplasmic 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin(2-19aaDDDIAALVVDNGSGMCKA), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-beta Actin Antikörper (ABIN3044573)

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Qiao, Wei, Dai, Zhou, Yin, Li, Xiao, Guo, Lu: "UCP2-related mitochondrial pathway participates in oroxylin A-induced apoptosis in human colon cancer cells." in: Journal of cellular physiology, Vol. 230, Issue 5, pp. 1054-63, (2015) (PubMed).

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Zheng, Ding, Ni, Song, Huang, Wang: "MiR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1." in: Oncology reports, Vol. 33, Issue 3, pp. 1291-9, (2015) (PubMed).

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Gong, Liu, Nie, Li, Xie, Liu, Liu, Mei, Li: "MiR-21/RASA1 axis affects malignancy of colon cancer cells via RAS pathways." in: World journal of gastroenterology, Vol. 21, Issue 5, pp. 1488-97, (2015) (PubMed).

Wang, Li, Tang, Yang, Wang, Liu, Yang, Yang: "Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma." in: Oncology reports, Vol. 33, Issue 4, pp. 1807-14, (2015) (PubMed).

Fu, Lin, Liang, Hu: "Effects of recombinant baculovirus AcMNPV-BmK IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells." in: Cytotechnology, (2015) (PubMed).

Wang, Gai, Xu, Zheng, Lv, Li, Liu: "Shikonin protects chondrocytes from interleukin-1beta-induced apoptosis by regulating PI3K/Akt signaling pathway." in: International journal of clinical and experimental pathology, Vol. 8, Issue 1, pp. 298-308, (2015) (PubMed).

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Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: ACTB (ACTB Produkte)

Synonyme: BRWS1 antikoerper, PS1TP5BP1 antikoerper, ACTA1 antikoerper, Actx antikoerper, E430023M04Rik antikoerper, beta-actin antikoerper, ps1tp5bp1 antikoerper, act-2 antikoerper, actb antikoerper, B-actin antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, A antikoerper, ACT5C antikoerper, Ac5C antikoerper, Act antikoerper, Act-5C antikoerper, Act5 antikoerper, Act5c antikoerper, Actin antikoerper, Actin/BAP47 antikoerper, Actin5C antikoerper, BAP47 antikoerper, Bap47 antikoerper, CG4027 antikoerper, Dmel\\CG4027 antikoerper, M32055 antikoerper, T11 antikoerper, act antikoerper, act 5C antikoerper, act5C antikoerper, actin antikoerper, actin5C antikoerper, anon-EST:fe2D2 antikoerper, beta-actin/Bap47 antikoerper, cyt5C antikoerper, l(1)G0009 antikoerper, l(1)G0010 antikoerper, l(1)G0025 antikoerper, l(1)G0079 antikoerper, l(1)G0117 antikoerper, l(1)G0177 antikoerper, l(1)G0245 antikoerper, l(1)G0330 antikoerper, l(1)G0420 antikoerper, l(1)G0486 antikoerper, Bact antikoerper, ACT-5 antikoerper, ACTB antikoerper, B-ACTZF antikoerper, actba antikoerper, bact antikoerper, bactin1 antikoerper, bactzf antikoerper, wu:fd18f05 antikoerper, A26C1B antikoerper, POTE2alpha antikoerper, POTEACTIN antikoerper, Beta-actin antikoerper, actin beta antikoerper, actin, beta antikoerper, cytoplasmic actin OlCA1 antikoerper, beta-actin antikoerper, Actin 5C antikoerper, actin gamma 1 antikoerper, actin, beta 1 antikoerper, actin, beta L homeolog antikoerper, beta actin antikoerper, POTE ankyrin domain family member F antikoerper, actin, beta like 2 antikoerper, actin, cytoplasmic 1 antikoerper, ACTB antikoerper, Actb antikoerper, actb antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, Act5C antikoerper, ACTG1 antikoerper, actb1 antikoerper, actb.L antikoerper, LOC100136352 antikoerper, POTEF antikoerper, ACTBL2 antikoerper, LOC109073280 antikoerper

Hintergrund: Beta-Actin(ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Synonyms: A26C1A antibody|A26C1B antibody|ACTB antibody|ACTB_HUMAN antibody|Actin cytoplasmic 1 antibody|Actx antibody|b-actin antibody|beta cytoskeletal actin antibody|Beta-actin antibody|E430023M04Rik antibody|MGC128179 antibody|PS1TP5 binding protein 1 antibody|PS1TP5BP1 antibody

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction