beta Actin Antikörper (N-Term)

Produktnummer ABIN3044573

Der Kaninchen Polyklonal Anti-beta Actin-Antikörper wurde für WB validiert. Er ist geeignet, beta Actin in Proben von Human, Maus, Ratte, Huhn, Affe und Zebrafisch (Danio rerio) zu detektieren. Es sind 131+ Publikationen verfügbar.

Kurzübersicht für beta Actin Antikörper (N-Term) (ABIN3044573)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-beta Actin Antikörper

Bindungsspezifität: AA 2-19, N-Term

Verwendungszweck: Anti-beta Actin/ACTB Antibody Picoband®

Sequenz: DDDIAALVVD NGSGMCKA

Kreuzreaktivität (Details): No cross reactivity with other proteins

Produktmerkmale: Anti-beta Actin/ACTB Antibody (ABIN3044573). Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H., Kedes, L. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Molec. Cell. Biol. 3: 787-795, 1983. 2. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 3. Ueyama, H., Inazawa, J., Nishino, H., Ohkubo, I., Miwa, T. FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes.Cytogenet. Cell Genet. 74: 221-224, 1996.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-beta Actin Antikörper (ABIN3044573)

Zhai, Liu, Wang, Zhang, Li, Guo: "Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174414, (2017) (PubMed).

Chang, Zhang, Lihua, Feng: "T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism." in: American journal of translational research, Vol. 8, Issue 11, pp. 4694-4704, (2016) (PubMed).

Wang, Qi, Li, Wu, Wang, Wang, Ning: "Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells." in: International journal of immunopathology and pharmacology, Vol. 28, Issue 3, pp. 308-17, (2015) (PubMed).

Zuo, Liu, Zhang, Wu, Guo, Liao: "Development of trastuzumab-resistant human gastric carcinoma cell lines and mechanisms of drug resistance." in: Scientific reports, Vol. 5, pp. 11634, (2015) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Wang, Wu, Wang, Tian, Chen, Li: "The biophysical property of A549 cells transferred by VEGF-D." in: Scanning, Vol. 36, Issue 2, pp. 202-8, (2015) (PubMed).

Lee, Lee, Huo, Wang, Huang, Lin, Chuang, Lee: "Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 35, Issue 3, pp. 704-12, (2015) (PubMed).

Qiao, Wei, Dai, Zhou, Yin, Li, Xiao, Guo, Lu: "UCP2-related mitochondrial pathway participates in oroxylin A-induced apoptosis in human colon cancer cells." in: Journal of cellular physiology, Vol. 230, Issue 5, pp. 1054-63, (2015) (PubMed).

Wang, Wei, Wang, Du, Zhang, Zhou: "Cellular activation, expression analysis and functional characterization of grass carp I?B?: evidence for its involvement in fish NF-?B signaling pathway." in: Fish & shellfish immunology, Vol. 42, Issue 2, pp. 408-12, (2015) (PubMed).

Zheng, Ding, Ni, Song, Huang, Wang: "MiR-142 acts as a tumor suppressor in osteosarcoma cell lines by targeting Rac1." in: Oncology reports, Vol. 33, Issue 3, pp. 1291-9, (2015) (PubMed).

Shi, Yang, Mao, Wu, Ren, Yang, Yin, Wei, Li, Wang: "Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation." in: Acta pharmacologica Sinica, Vol. 36, Issue 2, pp. 252-8, (2015) (PubMed).

Pan, Chou, Sun: "P2X7 R-mediated Ca(2+) -independent d-serine release via pannexin-1 of the P2X7 R-pannexin-1 complex in astrocytes." in: Glia, Vol. 63, Issue 5, pp. 877-93, (2015) (PubMed).

Gong, Liu, Nie, Li, Xie, Liu, Liu, Mei, Li: "MiR-21/RASA1 axis affects malignancy of colon cancer cells via RAS pathways." in: World journal of gastroenterology, Vol. 21, Issue 5, pp. 1488-97, (2015) (PubMed).

Wang, Li, Tang, Yang, Wang, Liu, Yang, Yang: "Overexpression of Tiam1 promotes the progression of laryngeal squamous cell carcinoma." in: Oncology reports, Vol. 33, Issue 4, pp. 1807-14, (2015) (PubMed).

Fu, Lin, Liang, Hu: "Effects of recombinant baculovirus AcMNPV-BmK IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells." in: Cytotechnology, (2015) (PubMed).

Wang, Gai, Xu, Zheng, Lv, Li, Liu: "Shikonin protects chondrocytes from interleukin-1beta-induced apoptosis by regulating PI3K/Akt signaling pathway." in: International journal of clinical and experimental pathology, Vol. 8, Issue 1, pp. 298-308, (2015) (PubMed).

Rong, Ding, Zhang, Guo: "Neuregulin1? improves cognitive dysfunction and up-regulates expression of p-ERK1/2 in rats with chronic omethoate poisoning." in: Behavioral and brain functions : BBF, Vol. 11, pp. 5, (2015) (PubMed).

Yang, Yan, Liu, Wang, Hu: "Protective effects of acteoside against X?ray?induced damage in human skin fibroblasts." in: Molecular medicine reports, Vol. 12, Issue 2, pp. 2301-6, (2015) (PubMed).

Shi, Wu, Wang, Zhou, Yan, Chen, Gu, Cai: "Liquiritigenin Potentiates the Inhibitory Effects of Cisplatin on Invasion and Metastasis Via Downregulation MMP-2/9 and PI3 K/AKT Signaling Pathway in B16F10 Melanoma Cells and Mice Model." in: Nutrition and cancer, Vol. 67, Issue 5, pp. 761-70, (2015) (PubMed).

Zhao, Wang, Meng, Ji, Xu, Chen, Fan, Yu, Yao, Dong: "MicroRNA-27a-3p Inhibits Melanogenesis in Mouse Skin Melanocytes by Targeting Wnt3a." in: International journal of molecular sciences, Vol. 16, Issue 5, pp. 10921-33, (2015) (PubMed).

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: ACTB

Hintergrund:

Synonyms: Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;ACTB;

Tissue Specificity: Brain specific. .

Background: beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Sequence Similarities: Belongs to the actin family.

Molekulargewicht: 42 kDa

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction