Aromatase Antikörper (C-Term)

Produktnummer ABIN3044414

Produktdetails anti-Aromatase Antikörper

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Bindungsspezifität: AA 485-503, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aromatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Aromatase(CYP19A1) detection. Tested with WB, IHC-P in Human.

Sequenz: KNMLEMIFTP RNSDRCLEH

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Aromatase(CYP19A1) detection. Tested with WB, IHC-P in Human.
Gene Name: cytochrome P450, family 19, subfamily A, polypeptide 1
Protein Name: Aromatase

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aromatase(485-503aa KNMLEMIFTPRNSDRCLEH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Aromatase Antikörper (ABIN3044414)

Zhang, Zhang, Zhang, Lu, Li, Cui: "MiRNA-143 mediates the proliferative signaling pathway of FSH and regulates estradiol production." in: The Journal of endocrinology, Vol. 234, Issue 1, pp. 1-14, (2017) (PubMed).

Zhang, Yang, Zou, Lu, Xiong, Wu, Wang, Gao, Xu, Zou: "Estradiol synthesis and release in cultured female rat bone marrow stem cells." in: BioMed research international, Vol. 2013, pp. 301540, (2013) (PubMed).

Zhao, Tian, Hao, Chen: "Extragonadal aromatization increases with time after ovariectomy in rats." in: Reproductive biology and endocrinology : RB&E, Vol. 3, pp. 6, (2006) (PubMed).

Zhao, Tian, Chen: "Electroacupuncture stimulates hypothalamic aromatization." in: Brain research, Vol. 1037, Issue 1-2, pp. 164-70, (2005) (PubMed).

Details zu Aromatase

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Andere Bezeichnung: CYP19A1 (CYP19A1 Produkte)

Hintergrund: Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

Synonyms: ARO 1 antibody|ARO antibody|ARO1 antibody|Aromatase antibody|CP19A_HUMAN antibody|CPV antibody|CPV1 antibody|CYAR antibody|CYP19 antibody|CYP19A1 antibody|CYPXIX antibody|Cytochrome P-450AROM antibody|Cytochrome P450 19A1 antibody|Cytochrome P450 family 19 subfamily A polypeptide 1 antibody|Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily XIX(aromatization of androgens) antibody|Estrogen synthase antibody|Estrogen synthetase antibody|Flavoprotein linked monooxygenase antibody|MGC104309 antibody|Microsomal monooxygenase antibody|OTTHUMP00000162543 antibody|OTTHUMP00000198350 antibody|P 450AROM antibody|P450AROM antibody

UniProt: P11511

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis