DLD Antikörper (C-Term)

Produktnummer ABIN3044405

Produktdetails anti-DLD Antikörper

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Bindungsspezifität: AA 492-509, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DLD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: EAFREANLAA SFGKSINF

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: dihydrolipoamide dehydrogenase
Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DLD(492-509aa EAFREANLAASFGKSINF), different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Andere Bezeichnung: DLD (DLD Produkte)

Synonyme: DLDD antikoerper, DLDH antikoerper, E3 antikoerper, GCSL antikoerper, LAD antikoerper, PHE3 antikoerper, AI315664 antikoerper, AI746344 antikoerper, wu:fb24b05 antikoerper, DLD antikoerper, DDBDRAFT_0183800 antikoerper, DDBDRAFT_0216232 antikoerper, DDB_0183800 antikoerper, DDB_0216232 antikoerper, sc:d0402 antikoerper, dihydrolipoamide dehydrogenase antikoerper, dihydrolipoyl dehydrogenase antikoerper, deltaD antikoerper, DLD antikoerper, Dld antikoerper, dldh antikoerper, AT4G16155 antikoerper, CND05840 antikoerper, bfmBC antikoerper, GCSL antikoerper, LACBIDRAFT_182385 antikoerper, UREG_06178 antikoerper, lpd antikoerper, TAGG_RS02070 antikoerper, Arnit_2606 antikoerper, Mesil_1945 antikoerper, Trad_2118 antikoerper, Acear_0640 antikoerper, Fbal_0372 antikoerper, Ilyop_1890 antikoerper, Ftrac_1733 antikoerper, Ocepr_1753 antikoerper, Intca_2017 antikoerper, Deima_0504 antikoerper, dld antikoerper

Hintergrund: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex(BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Synonyms: Diaphorase antibody|Dihydrolipoamide dehydrogenase antibody|Dihydrolipoyl dehydrogenase antibody|Dihydrolipoyl dehydrogenase mitochondrial antibody|dld antibody|DLDH antibody|DLDH_HUMAN antibody|E3 antibody|E3 component of pyruvate dehydrogenase antibody|E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody|GCSL antibody|Glycine cleavage system L protein antibody|Glycine cleavage system protein L antibody|LAD antibody|lipoamide dehydrogenase antibody|Lipoamide reductase antibody|Lipoyl dehydrogenase antibody|mitochondrial antibody|OTTHUMP00000206744 antibody|OTTHUMP00000206746 antibody|OTTHUMP00000206748 antibody|OTTHUMP00000206749 antibody|PHE 3 antibody|PHE3 antibody

UniProt: P09622

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis