PPARG Antikörper (N-Term)

Produktnummer ABIN3044366

Der Kaninchen Polyklonal Anti-PPARG-Antikörper wurde für WB validiert. Er ist geeignet, PPARG in Proben von Human zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für PPARG Antikörper (N-Term) (ABIN3044366)

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPARG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PPARG Antikörper

Bindungsspezifität: AA 45-62, N-Term

Verwendungszweck: Anti-PPAR gamma/PPARG Antibody Picoband®

Sequenz: PHYEDIPFTR TDPVVADY

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-PPAR gamma/PPARG Antibody (ABIN3044366). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma, different from the rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. Berger J, Moller DE (2002). "The mechanisms of action of PPARs". Annu. Rev. Med. 53: 409-35. 2. Deeb, S. S., Fajas, L., Nemoto, M., Pihlajamaki, J., Mykkanen, L., Kuusisto, J., Laakso, M., Fujimoto, W., Auwerx, J. : A pro12ala substitution in PPAR-gamma-2 associated with decreased receptor activity, lower body mass index and improved insulin sensitivity. Nature Genet. 20: 284-287, 1998. 3. Ameshima, S., Golpon, H., Cool, C. D., Chan, D., Vandivier, R. W., Gardai, S. J., Wick, M., Nemenoff, R. A., Geraci, M. W., Voelkel, N. F. : Peroxisome proliferator-activated receptor gamma (PPAR-gamma) expression is decreased in pulmonary hypertension and affects endothelial cell growth. Circ. Res. 92: 1162-1169, 2003.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-PPARG Antikörper (ABIN3044366)

Wu, Wu, Jiao, Wang, Wang, Zhang: "Rosiglitazone infusion therapy following minimally invasive surgery for intracerebral hemorrhage evacuation decreases matrix metalloproteinase-9 and blood-brain barrier disruption in rabbits." in: BMC neurology, Vol. 15, pp. 37, (2016) (PubMed).

Jiang, Zhang, Zhang, Zhu, Li, Lu, Xu, Chen, Lin: "Pravastatin prevents steroid-induced osteonecrosis in rats by suppressing PPAR? expression and activating Wnt signaling pathway." in: Experimental biology and medicine (Maywood, N.J.), Vol. 239, Issue 3, pp. 347-55, (2014) (PubMed).

Chen, Bai, Liao, Peng, Wu, Wang, Zeng, Xie: "Electrospun poly(L-lactide)/poly(?-caprolactone) blend nanofibrous scaffold: characterization and biocompatibility with human adipose-derived stem cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e71265, (2013) (PubMed).

Wang, Liu, Dang, Ma, Zhang, Wang: "The effect of core decompression on local expression of BMP-2, PPAR-γ and bone regeneration in the steroid-induced femoral head osteonecrosis." in: BMC musculoskeletal disorders, Vol. 13, pp. 142, (2012) (PubMed).

Xie, Wang, Zhang, Li, Can, Qing, Kung, Zhang: "Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice." in: Experimental and therapeutic medicine, Vol. 2, Issue 2, pp. 309-315, (2012) (PubMed).

Xie, Zhao, Gu, Du, Cai, Zhang: "Scorpion in Combination with Gypsum: Novel Antidiabetic Activities in Streptozotocin-Induced Diabetic Mice by Up-Regulating Pancreatic PPARγ and PDX-1 Expressions." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2011, pp. 683561, (2011) (PubMed).

Details zu PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Andere Bezeichnung: PPARG

Hintergrund:

Synonyms: Peroxisome proliferator-activated receptor gamma,PPAR-gamma,Nuclear receptor subfamily 1 group C member 3,PPARG,NR1C3,

Tissue Specificity: Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. .

Background: The peroxisome proliferator-activated receptors (PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al. (2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.

Sequence Similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.

Molekulargewicht: 37 kDa

UniProt: P37231

Pathways: MAPK Signalweg, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation