Decorin Antikörper (C-Term)

Produktnummer ABIN3044362

Produktdetails anti-Decorin Antikörper

Target: Decorin (DCN)

Bindungsspezifität: AA 343-359, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Decorin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-Decorin/DCN Antibody Picoband®

Sequenz: TFRCVYVRSA IQLGNYK

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Decorin/DCN Antibody (ABIN3044362). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Decorin, identical to the related mouse sequence, and different from the related rat sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Danielson, K. G., Fazzio, A., Cohen, I., Cannizzaro, L. A., Eichstetter, I., Iozzo, R. V. : The human decorin gene: intron-exon organization, discovery of two alternatively spliced exons in the 5-prime untranslated region, and mapping of the gene to chromosome 12q23. Genomics 15: 146-160, 1993. 2. Vetter, U., Vogel, W., Just, W., Young, M. F., Fisher, L. W. : Human decorin gene: intron-exon junctions and chromosomal localization. Genomics 15: 161-168, 1993. 3. Scholzen, T., Solursh, M., Suzuki, S., Reiter, R., Morgan, J. L., Buchberg, A. M., Siracusa, L. D., Iozzo, R. V. : The murine decorin: complete cDNA cloning, genomic organization, chromosomal assignment, and expression during organogenesis and tissue differentiation. J. Biol. Chem. 269: 28270-28281, 1994.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Decorin Antikörper (ABIN3044362)

Han, Wang, Jiang, Ma, Tian, Zang, Ma: "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." in: Scientific reports, Vol. 6, pp. 35102, (2016) (PubMed).

Huang, Zou, Shi, Zhang, Pen, Zhang, Gao, Wang: "The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 731395, (2014) (PubMed).

Details zu Decorin

Target: Decorin (DCN)

Andere Bezeichnung: DCN (DCN Produkte)

Hintergrund:

Synonyms: Decorin,Bone proteoglycan II,PG-S2,PG40,DCN,SLRR1B,

Tissue Specificity: Ubiquitously expressed. .

Background: Decorin is a proteoglycan on average 90-140 kilodaltons (kD) in size. The human decorin gene spans more than 38 kb and contains 8 exons and very large introns, 2 of which are 5.4 and more than 13.2 kb. And the human decorin gene is mapped to 12q21.3. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are though to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. And it also may play a role in epithelial/mesenchymal interactions during organ development and shaping. Decorin has been shown to have anti-tumorigenic properties in an experimental murine tumor model and is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for the decorin gene. Mutations in the decorin gene are associated with congenital stromal corneal dystrophy.

Sequence Similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Molekulargewicht: 40-50 kDa, 80-140 kDa

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process