Cullin 4B Antikörper (C-Term)

Produktnummer ABIN3044348

Der Kaninchen Polyklonal Anti-Cullin 4B-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Cullin 4B in Proben von Human, Maus, Ratte und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für Cullin 4B Antikörper (C-Term) (ABIN3044348)

Target: Cullin 4B (CUL4B)

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cullin 4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Cullin 4B Antikörper

Bindungsspezifität: AA 895-913, C-Term

Verwendungszweck: Anti-Cullin 4B/CUL4B Antibody Picoband®

Sequenz: DRDYMERDKE NPNQYNYIA

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cullin 4B/CUL4B Antibody (ABIN3044348). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Zebrafish
Western blot, 0.1-0.5 μg/mL, Human, Rat, Zebrafish, Mouse
1. Zou, Y., Liu, Q., Chen, B., Zhang, X., Guo, C., Zhou, H., Li, J., Gao, G., Guo, Y., Yan, C., Wei, J., Shao, C., Gong, Y. : Mutation in CUL4B, which encodes a member of cullin-RING ubiquitin ligase complex, causes X-linked mental retardation. Am. J. Hum. Genet. 80: 561-566, 2007. 2. Tarpey, P. S., Raymond, F. L., O'Meara, S., Edkins, S., Teague, J., Butler, A., Dicks, E., Stevens, C., Tofts, C., Avis, T., Barthorpe, S., Buck, G. : {and 41 others}: Mutations in CUL4B, which encodes a ubiquitin E3 ligase subunit, cause an X-linked mental retardation syndrome associated with aggressive outbursts, seizures, relative macrocephaly, central obesity, hypogonadism, pes cavus, and tremor. Am. J. Hum. Genet. 80: 345-352, 2007. 3. Ohtake, F., Baba, A., Takada, I., Okada, M., Iwasaki, K., Miki, H., Takahashi, S., Kouzmenko, A., Nohara, K., Chiba, T., Fujii-Kuriyama, Y., Kato, S. : Dioxin receptor is a ligand-dependent E3 ubiquitin ligase. Nature 446: 562-566, 2007.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Cullin 4B

Target: Cullin 4B (CUL4B)

Andere Bezeichnung: CUL4B

Hintergrund:

Synonyms: Cullin-4B,CUL-4B,CUL4B,KIAA0695,

Tissue Specificity: Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Background: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B (AhR). Complex assembly and ubiquitin ligase activity of CUL4B (AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B (AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

Sequence Similarities: Belongs to the cullin family.

Molekulargewicht: 104 kDa

UniProt: Q13620