Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) Antikörper

Produktnummer ABIN3044322

Produktdetails

Target: Deoxyuridine Triphosphatase (DUT)

Bindungsspezifität: AA 212-229, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial(DUT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: KKGDRIAQLI CERIFYPE

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial(DUT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: deoxyuridine triphosphatase
Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DUT(212-229aa KKGDRIAQLICERIFYPE), different from the related rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT (DUT Produkte)

Synonyme: BcDNA:LD08534 antikoerper, CG4584 antikoerper, Dmel\\CG4584 antikoerper, LD08534 antikoerper, UTPase antikoerper, anon-SAGE:Wang-077 antikoerper, dUTPase antikoerper, Tb07.27E10.390 antikoerper, dutpase antikoerper, 5031412I06Rik antikoerper, 5133400F09Rik antikoerper, D2Bwg0749e antikoerper, Dutp antikoerper, PIP4 antikoerper, deoxyuridine triphosphatase antikoerper, Deoxyuridine triphosphatase antikoerper, dUTPase; ORF54; similar to EBV BLLF3, CMV UL72, HSV UL50 antikoerper, involved in nucleotide metabolism antikoerper, tegument protein antikoerper, dUTPase antikoerper, deoxyuridine triphosphatase L homeolog antikoerper, DUT antikoerper, dUTPase antikoerper, P18 antikoerper, AlHV1gp51 antikoerper, UL50 antikoerper, HVT058 antikoerper, ORF9 antikoerper, ORF8 antikoerper, U45 antikoerper, GAMMAHV.ORF54 antikoerper, Tc00.1047053509151.130 antikoerper, Tc00.1047053508175.160 antikoerper, Tb927.7.5160 antikoerper, dut.L antikoerper, Dut antikoerper

Substanzklasse: Viral Protein

Hintergrund: Deoxyuridine triphosphate nucleotidohydrolase(dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35 % identity with the E.coli dUTPase and 53 % identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92 % identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Synonyms: Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform antibody|Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial antibody|Deoxyuridine triphosphatase antibody|dut antibody|DUT_HUMAN antibody|dUTP nucleotidohydrolase antibody|dUTP pyrophosphatase antibody|dUTPase antibody|FLJ20622 antibody

UniProt: P33316