MCM3 Antikörper (N-Term)
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- Target Alle MCM3 Antikörper anzeigen
- MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))
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Bindungsspezifität
- AA 13-27, N-Term
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser MCM3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- Verwendungszweck
- Anti-MCM3 Antibody Picoband®
- Sequenz
- REAQRDYLDF LDDEE
- Kreuzreaktivität (Details)
- No cross-reactivity with other proteins
- Produktmerkmale
- Anti-MCM3 Antibody (ABIN3044243). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Aufreinigung
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3, identical to the related rat and mouse sequences.
- Isotyp
- IgG
- Top Product
- Discover our top product MCM3 Primärantikörper
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- Applikationshinweise
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Thommes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (Apr 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res 20 (5): 1069-74. 3. "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)". - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. - Haltbarkeit
- 12 months
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- Target
- MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))
- Andere Bezeichnung
- MCM3 (MCM3 Produkte)
- Hintergrund
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Synonyms: DNA replication licensing factor MCM3,3.6.4.12,DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102,MCM3,
Tissue Specificity: Heart, liver, duodenum, blood vessels and kidney.
Background: MCM3 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact ly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Sequence Similarities: Belongs to the MCM family.
- Molekulargewicht
- 100-110 kDa
- UniProt
- P25205
- Pathways
- DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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