GRP78 Antikörper (C-Term)

Produktnummer ABIN3044191

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GRP78 in WB, IHC und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für GRP78 Antikörper (C-Term) (ABIN3044191)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-GRP78 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-GRP78 BiP/HSPA5 Antibody

Sequenz: EWLESHQDAD IEDFK

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: identical to the related mouse,rat sequences.

Produktmerkmale: Anti-GRP78 BiP/HSPA5 Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GRP78 Antikörper (ABIN3044191)

Wang, Wang, Huang, Yang, Zhao, Wei, Zhao: "Erratum to: Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways." in: Heart and vessels, Vol. 32, Issue 2, pp. 216, (2016) (PubMed).

Wu, Dong, Li: "Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload." in: International journal of molecular medicine, Vol. 35, Issue 4, pp. 893-900, (2015) (PubMed).

Li, Zhao, Xing, Sun: "Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning." in: Neural regeneration research, Vol. 10, Issue 3, pp. 467-72, (2015) (PubMed).

Ding, Zou, Li, Tian, Abdelalim, Du, She, Wang, Tan, Wang, Chen, Lv, Chang: "Study of histopathological and molecular changes of rat kidney under simulated weightlessness and resistance training protective effect." in: PLoS ONE, Vol. 6, Issue 5, pp. e20008, (2011) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: HSPA5

Hintergrund:

Background: HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al. (2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER.

Gene Full Name: heat shock protein family A (Hsp70) member 5

Sequence Similarities: Belongs to the heat shock protein 70 family.

Molekulargewicht: 70 kDa

Gen-ID: 3309

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling