YBX1 Antikörper (Middle Region)

Produktnummer ABIN3044148

Dieses Anti-YBX1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von YBX1 in WB, IHC und FACS. Geeignet für Human, Ratte und Maus.

Kurzübersicht für YBX1 Antikörper (Middle Region) (ABIN3044148)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YBX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-YBX1 Antikörper

Bindungsspezifität: AA 137-152, Middle Region

Verwendungszweck: Anti-YB1/YBX1 Antibody Picoband®

Sequenz: KYAADRNHYR RYPRRR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-YB1/YBX1 Antibody (ABIN3044148). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human YB1, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bhullar, J., Sollars, V. E. YBX1 expression and function in early hematopoiesis and leukemic cells. Immunogenetics 63: 337-350, 2011. 2. Fukada, T., Tonks, N. K. Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling. EMBO J. 22: 479-493, 2003. 3. Makino, Y., Ohga, T., Toh, S., Koike, K., Okumura, K., Wada, M., Kuwano, M., Kohno, K. Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter. Nucleic Acid Res. 24: 1873-1878, 1996.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Andere Bezeichnung: YBX1

Hintergrund:

Synonyms: Nuclease-sensitive element-binding protein 1,CCAAT-binding transcription factor I subunit A,CBF-A,DNA-binding protein B,DBPB,Enhancer factor I subunit A,EFI-A,Y-box transcription factor,Y-box-binding protein 1,YB-1,YBX1,NSEP1, YB1,

Tissue Specificity: In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. .

Background: YBX1 (Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18 % basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.

Sequence Similarities: Contains 1 CSD (cold-shock) domain.

Molekulargewicht: 50 kDa

UniProt: P67809

Pathways: Regulation of Muscle Cell Differentiation