IL-4 Antikörper (Middle Region)

Produktnummer ABIN3044141

Der Kaninchen Polyklonal Anti-IL-4-Antikörper wurde für WB und IHC validiert. Er ist geeignet, IL-4 in Proben von Human zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für IL-4 Antikörper (Middle Region) (ABIN3044141)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-IL-4 Antikörper

Bindungsspezifität: AA 72-88, Middle Region

Verwendungszweck: Anti-Interleukin-4 IL4 Antibody Picoband®

Sequenz: AATVLRQFYS HHEKDTR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Interleukin-4 IL4 Antibody (ABIN3044141). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human IL-4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Prokopchuk, O., Liu, Y., Henne-Bruns, D., Kornmann, M. Interleukin-4 enhances proliferation of human pancreatic cancer cells: evidence for autocrine and paracrine actions. Br J Cancer.2005 Mar 14,92(5):921-8. 2. Arai, N., Nomura, D., Villaret, D., DeWaal Malefijt, R., Seiki, M., Yoshida, M., Minoshima, S., Fukuyama, R., Maekawa, M., Kudoh, J., Shimizu, N., Yokota, K., Abe, E., Yokota, T., Takebe, Y., Arai, K. Complete nucleotide sequence of the chromosomal gene for human IL-4 and its expression. J. Immun. 142: 274-282, 1989.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-IL-4 Antikörper (ABIN3044141)

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Yan, Wang, Liang, Fu, Guo: "HPV16L1-attenuated Shigella recombinant vaccine induced strong vaginal and systemic immune responses in guinea pig model." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 12, pp. 3491-8, (2015) (PubMed).

Wang, Feng, Shi, Hao, Yang, Zhang, Kong: "Silencing of miR155 promotes the production of inflammatory mediators in Guillain-Barré syndrome in vitro." in: Inflammation, Vol. 36, Issue 2, pp. 337-45, (2013) (PubMed).

Tan, Liu, Yang, Wang, Qin: "An inactivated Pseudomonas aeruginosa medicament inhibits airway allergic inflammation and improves epithelial functions." in: The journal of physiological sciences : JPS, Vol. 63, Issue 1, pp. 63-9, (2012) (PubMed).

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL4

Hintergrund:

Synonyms: Interleukin-4,IL-4,B-cell stimulatory factor 1,BSF-1,Binetrakin,Lymphocyte stimulatory factor 1,Pitrakinra,IL4,

Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Sequence Similarities: Belongs to the IL-4/IL-13 family.

Molekulargewicht: 14 kDa

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation