IGF1R Antikörper (C-Term)

Produktnummer ABIN3044138

Produktdetails anti-IGF1R Antikörper

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Bindungsspezifität: AA 1353-1367, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGF1R Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Insulin-like growth factor 1 receptor(IGF1R) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: RKNERALPLP QSSTC

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Insulin-like growth factor 1 receptor(IGF1R) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: insulin-like growth factor 1 receptor
Protein Name: Insulin-like growth factor 1 receptor

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IGF1 Receptor(1353-1367aa RKNERALPLPQSSTC), different from the related rat and mouse sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-IGF1R Antikörper (ABIN3044138)

Lai, Chen, Chen, Ruan, He, Chen, Tang, Wang: "Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling." in: Scientific reports, Vol. 7, pp. 44473, (2018) (PubMed).

Liu, Ma, Li, Yu, Zhou, Lu, Jin, Wang, Yu: "Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways." in: Experimental & molecular medicine, Vol. 50, Issue 4, pp. 25, (2018) (PubMed).

Ramdhave, Ojha, Nandave: "Energy intake correlates with the levels of fatty acid synthase and insulin-like growth factor-1 in male and female C57BL/6 mice." in: American journal of translational research, Vol. 9, Issue 3, pp. 830-844, (2017) (PubMed).

Zhang, Zhang, Yu, Du, Liu, Liu, An, Wang, Song, Li, Cao: "Effects of interferon tau on endometrial epithelial cells in caprine in vitro." in: Gene expression patterns : GEP, Vol. 25-26, pp. 142-148, (2017) (PubMed).

Li, Chen, Liu, Hou: "Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway." in: World journal of gastroenterology, Vol. 22, Issue 23, pp. 5353-63, (2017) (PubMed).

Hou, Wan, Wang, Li, Wang, Yao, Feng, Jing, Lu, Jia, Peng: "Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression." in: American journal of translational research, Vol. 8, Issue 9, pp. 3656-3665, (2016) (PubMed).

Liang, Hao, Zhang, Wu, Wang: "Insulin-like growth factors in endometrioid adenocarcinoma: correlation with clinico-pathological features and estrogen receptor expression." in: BMC cancer, Vol. 12, pp. 262, (2013) (PubMed).

Zhang, Leng, Wang, Zhang: "Treating human meniscal fibrochondrocytes with hIGF-1 gene by liposome." in: Clinical orthopaedics and related research, Vol. 467, Issue 12, pp. 3175-82, (2010) (PubMed).

Details zu IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Andere Bezeichnung: IGF1R (IGF1R Produkte)

Synonyme: CMT2N antikoerper, CD221 antikoerper, IGFIR antikoerper, IGFR antikoerper, JTK13 antikoerper, IGFIRC antikoerper, IGFR1 antikoerper, IGF-1R antikoerper, IGF1R antikoerper, DKFZp469I0618 antikoerper, igf-1r antikoerper, xIGF-1R antikoerper, xIGFR antikoerper, xigf1r antikoerper, A330103N21Rik antikoerper, D930020L01 antikoerper, hyft antikoerper, igf-ir antikoerper, IGF-IR antikoerper, IGF-IRb antikoerper, wu:fb03h06 antikoerper, IGF-IRa antikoerper, sb:eu1090 antikoerper, wu:fi20b10 antikoerper, alanyl-tRNA synthetase antikoerper, insulin like growth factor 1 receptor antikoerper, insulin-like growth factor 1 receptor antikoerper, insulin-like growth factor 1 antikoerper, insulin-like growth factor I receptor antikoerper, insulin like growth factor 1 receptor S homeolog antikoerper, insulin-like growth factor 1b receptor antikoerper, insulin-like growth factor 1a receptor antikoerper, AARS antikoerper, IGF1R antikoerper, Igf1r antikoerper, igf1r antikoerper, Igf1 antikoerper, LOC780503 antikoerper, LOC100136427 antikoerper, igf1r.S antikoerper, igf1rb antikoerper, igf1ra antikoerper

Hintergrund: IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of phosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45 % of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.

Synonyms: CD221 antibody|CD221 antigen antibody|IGF 1 receptor antibody|IGF 1R antibody|IGF I receptor antibody|IGF-I receptor antibody|IGF1R antibody|IGF1R_HUMAN antibody|IGFIR antibody|IGFIRC antibody|IGFR antibody|Insulin like growth factor 1 receptor antibody|Insulin like growth factor 1 receptor precursor antibody|Insulin-like growth factor 1 receptor beta chain antibody|Insulin-like growth factor I receptor antibody|JTK13 antibody|MGC142170 antibody|MGC142172 antibody|MGC18216 antibody

UniProt: P08069

Pathways: RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie