CD2 Antikörper (Middle Region)

Produktnummer ABIN3044135

Der Kaninchen Polyklonal Anti-CD2-Antikörper wurde für WB validiert. Er ist geeignet, CD2 in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für CD2 Antikörper (Middle Region) (ABIN3044135)

Target: CD2

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CD2 Antikörper

Bindungsspezifität: AA 224-242, Middle Region

Verwendungszweck: Anti-CD2/CD2 Antibody Picoband®

Sequenz: FIFCICKRRK RNRRRKDEE

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-CD2/CD2 Antibody (ABIN3044135). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse CD2, different from the related rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Brown, M. H., Gorman, P. A., Sewell, W. A., Spurr, N. K., Sheer, D., Crumpton, M. J. The gene coding for the human T-lymphocyte CD2 antigen is located on chromosome 1p. Hum. Genet. 76: 191-195, 1987. 2. Clayton, L. K., Ramachandran, H., Pravtcheva, D., Chen, Y.-F., Diamond, D. J., Ruddle, F. H., Reinherz, E. L. The gene for T11 (CD2) maps to chromosome 1 in humans and to chromosome 3 in mice. J. Immun. 140: 3617-3621, 1988. 3. Kingsmore, S. F., Watson, M. L., Moseley, W. S., Seldin, M. F. Physical linkage of genes encoding the lymphocyte adhesion molecules CD2 and its ligand LFA-3. Immunogenetics 30: 123-125, 1989.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CD2

Target: CD2

Andere Bezeichnung: CD2

Hintergrund:

Synonyms: T-cell surface antigen CD2,LFA-2,LFA-3 receptor,Lymphocyte antigen 37,Ly-37,T-cell surface antigen T11/Leu-5,CD2,Cd2,Ly-37,

Background: CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies ed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95 % of thymocytes, it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily, it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al. (1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.

Sequence Similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Molekulargewicht: 65 kDa

UniProt: P08920