Beclin 1 Antikörper (Middle Region)

Produktnummer ABIN3044080

Produktdetails anti-Beclin 1 Antikörper

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Bindungsspezifität: AA 222-237, Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Beclin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Beclin-1(BECN1) detection. Tested with WB in Human,Mouse,Rat.

Sequenz: LDQEEAQYQR EYSEFK

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Beclin-1(BECN1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: beclin 1, autophagy related
Protein Name: Beclin-1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1(222-237aa LDQEEAQYQREYSEFK), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Beclin 1 Antikörper (ABIN3044080)

Qi, Xue, Lv, Sun, Du, Cai, Li, Gu, Wang: "Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway." in: Oncology letters, Vol. 15, Issue 5, pp. 6400-6408, (2018) (PubMed).

Shen, Cai, Liu, Li, Gan, Li, Wang, Guo, Zhou, Lu, Sun, Li: "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." in: Journal of hematology & oncology, Vol. 11, Issue 1, pp. 95, (2018) (PubMed).

Wang, Ma, Hu, Xie, Wu, Zeng, Song: "Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors." in: BMC cancer, Vol. 16, pp. 545, (2017) (PubMed).

Sun, Gao, Meng, Xu, Wang, Gu, Guo, Shao, Yan, Jiang, Zheng: "Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy." in: Frontiers in pharmacology, Vol. 8, pp. 524, (2017) (PubMed).

Bu, Zhao, Zhang, Wang, Li, Yan: "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." in: American journal of cancer research, Vol. 6, Issue 5, pp. 924-36, (2016) (PubMed).

Details zu Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Andere Bezeichnung: BECN1 (BECN1 Produkte)

Synonyme: beclin-1 antikoerper, atg6 antikoerper, vps30 antikoerper, beclin1 antikoerper, Beclin-1 antikoerper, becn1 antikoerper, zgc:73097 antikoerper, ATG6 antikoerper, VPS30 antikoerper, 4921513J16Rik antikoerper, 5430417M23Rik antikoerper, Atg6 antikoerper, beclin 1 antikoerper, beclin 1, autophagy related antikoerper, beclin 1 protein antikoerper, beclin-1 antikoerper, beclin family protein antikoerper, beclin 1, autophagy related L homeolog antikoerper, BECN1 antikoerper, becn1 antikoerper, atg6 antikoerper, CpipJ_CPIJ011087 antikoerper, PTRG_07825 antikoerper, SJAG_01876 antikoerper, Becn1 antikoerper, becn1.L antikoerper

Hintergrund: Bellini-1(beclin 1, autophagy related) is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene(Atg) 6. Beclin-1 and its binding partner class III phosphoinositide 3-kinase(PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. Human beclin encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed beclin immunoreactivity in many neurons throughout these regions, as well as in some glial cells. Beclin protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that beclin reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.

Synonyms: APG6 antibody|ATG 6 antibody|ATG6 antibody|ATG6 autophagy related 6 homolog antibody|Bcl-2-interacting protein beclin antibody|Beclin 1(coiled coil moesin like BCL2 interacting protein) antibody|Beclin 1 autophagy related antibody|Beclin-1 antibody|Beclin1 antibody|BECN 1 antibody|BECN1 antibody|BECN1_HUMAN antibody|Coiled coil myosin like BCL2 interacting protein antibody|Coiled-coil myosin-like BCL2-interacting protein antibody|GT197 antibody|Protein GT197 antibody|VPS 30 antibody|VPS30 antibody

UniProt: Q14457

Pathways: Autophagie