Annexin VII Antikörper (C-Term)

Produktnummer ABIN3044073

Produktdetails anti-Annexin VII Antikörper

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Bindungsspezifität: AA 445-460, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Annexin VII Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Verwendungszweck: Anti-Annexin VII/ANXA7 Antibody Picoband®

Sequenz: TRSEIDLVQI KQMFAQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Annexin VII/ANXA7 Antibody (ABIN3044073). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VII, different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Caohuy, H., Srivastava, M., Pollard, H. B. Membrane fusion protein synexin (annexin VII) as a Ca(2+)/GTP sensor in exocytotic secretion. Proc. Nat. Acad. Sci. 93: 10797-10802, 1996. 2. Shirvan, A., Srivastava, M., Wang, M. G., Cultraro, C., Magendzo, K., McBride, O. W., Pollard, H. B., Burns, A. L. Divergent structure of the human synexin (annexin VII) gene and assignment to chromosome 10. Biochemistry 33: 6888-6901, 1994. 3. Srivastava, M., Bubendorf, L., Srikantan, V., Fossom, L., Nolan, L., Glasman, M., Leighton, X., Fehrle, W., Pittaluga, S., Raffeld, M., Koivisto, P., Willi, N., Gasser, T. C., Kononen, J., Sauter, G., Kallioniemi, O. P., Srivastava, S., Pollard, H. B. ANX7, a candidate tumor suppressor gene for prostate cancer. Proc. Nat. Acad. Sci. 98: 4575-4580, 2001.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Annexin VII Antikörper (ABIN3044073)

Zhou, Li, Liu, Li, Wang, Shen: "Increased expression of annexin A7 in temporal lobe tissue of patients with refractory epilepsy." in: Histology and histopathology, Vol. 26, Issue 5, pp. 571-9, (2011) (PubMed).

Details zu Annexin VII

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Andere Bezeichnung: ANXA7 (ANXA7 Produkte)

Hintergrund:

Synonyms: Annexin A7,Annexin VII,Annexin-7,Synexin,ANXA7,ANX7, SNX,OK/SW-cl.95,

Tissue Specificity: Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. .

Background: ANXA7 (Annexin A7), also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al. (1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al. (2001) concluded that the ANX7 gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al. (1996) present experimental evidence, based on studies of recombinant human ANXA7 and isolated bovine chromaffin cells, that ANXA7 is a Ca (2+)-dependent GTP binding protein. ANXA7 was active in a chromaffin granule aggregation assays in the presence of Ca (2+) and GTP, and was deactivated upon GTP hydrolysis.

Sequence Similarities: Belongs to the annexin family.

Molekulargewicht: 50 kDa

UniProt: P20073