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MAP3K3 Antikörper (N-Term)

MAP3K3 Reaktivität: Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044008
  • Target Alle MAP3K3 Antikörper anzeigen
    MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))
    Bindungsspezifität
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 11-25, N-Term
    Reaktivität
    • 30
    • 27
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus, Ratte
    Wirt
    • 35
    • 4
    Kaninchen
    Klonalität
    • 28
    • 11
    Polyklonal
    Konjugat
    • 27
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MAP3K3 Antikörper ist unkonjugiert
    Applikation
    • 29
    • 14
    • 9
    • 9
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Verwendungszweck
    Anti-MEKK3/MAP3K3 Antibody Picoband®
    Sequenz
    MKDLVALQMS RRTRL
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-MEKK3/MAP3K3 Antibody (ABIN3044008). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3, different from the related rat sequence by two amino acids.
    Isotyp
    IgG
    Top Product
    Discover our top product MAP3K3 Primärantikörper
  • Applikationshinweise
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Mouse
    Western blot, 0.1-0.5 μg/mL, Mouse, Rat
    1. Channavajhala, P. L., Rao, V. R., Spaulding, V., Lin, L. L., Zhang, Y. G. hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappa-B pathways in inflammation. Biochem. Biophys. Res. Commun. 334: 1214-1218, 2005. 2. Puffenberger, E. G., Strauss, K. A., Ramsey, K. E., Craig, D. W., Stephan, D. A., Robinson, D. L., Hendrickson, C. L., Gottlieb, S., Ramsay, D. A., Siu, V. M., Heuer, G. G., Crino, P. B., Morton, D. H. Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5. Brain 130: 1929-1941, 2007. 3. Uhlik, M. T., Abell, A. N., Johnson, N. L., Sun, W., Cuevas, B. D., Lobel-Rice, K. E., Horne, E. A., Dell'Acqua, M. L., Johnson, G. L. Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock. Nature Cell Biol. 5: 1104-1110, 2003.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Konservierungsmittel
    Thimerosal (Merthiolate), Sodium azide
    Vorsichtsmaßnahmen
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))
    Andere Bezeichnung
    MAP3K3 (MAP3K3 Produkte)
    Hintergrund

    Synonyms: Mitogen-activated protein kinase kinase kinase 3,2.7.11.25,MAPK/ERK kinase kinase 3,MEK kinase 3,MEKK 3,Map3k3,Mekk3,

    Background: MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted ly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

    Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

    Molekulargewicht
    71 kDa
    UniProt
    Q61084
    Pathways
    MAPK Signalweg
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