Vitamin D Receptor Antikörper (C-Term)

Produktnummer ABIN3043960

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Vitamin D Receptor in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Vitamin D Receptor Antikörper (C-Term) (ABIN3043960)

Target: Vitamin D Receptor (VDR)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vitamin D Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Vitamin D Receptor Antikörper

Bindungsspezifität: AA 377-402, C-Term

Verwendungszweck: Anti-Vitamin D Receptor/VDR Antibody Picoband®

Sequenz: HLLYAKMIQK LADLRSLNEE HSKQYR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Vitamin D Receptor/VDR Antibody Picoband® (ABIN3043960). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VDR, different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Jurutka, P. W., Remus, L. S., Whitfield, G. K., Thompson, P. D., Hsieh, J.-C., Zitzer, H., Tavakkoli, P., Galligan, M. A., Dang, H. T. L., Haussler, C. A., Haussler, M. R. The polymorphic N terminus in human vitamin D receptor isoforms influences transcriptional activity by modulating interaction with transcription factor IIB. Molec. Endocr. 14: 401-420, 2000. 2. Labuda, M., Ross, M. V., Fujiwara, T. M., Morgan, K., Ledbetter, D., Hughes, M. R., Glorieux, F. H. Two hereditary defects related to vitamin D metabolism map to the same region of human chromosome 12q.II(Abstract) Cytogenet. Cell Genet. 58: 1978, 1991. 3. Shah, S., Islam, M. N., Dakshanamurthy, S., Rizvi, I., Rao, M., Herrell, R., Zinser, G., Valrance, M., Aranda, A., Moras, D., Norman, A., Welsh, J., Byers, S. W. The molecular basis of vitamin D receptor and beta-catenin crossregulation. Molec. Cell 21: 799-809, 2006. Note: Erratum: Molec. Cell 21: 148 only, 2006.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Vitamin D Receptor Antikörper (ABIN3043960)

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Gao, Wang, Yan, Zeng, Ma, Niu, Zhou, Jiang, Chen: "Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats." in: PLoS ONE, Vol. 11, Issue 3, pp. e0151118, (2016) (PubMed).

Details zu Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Andere Bezeichnung: VDR

Substanzklasse: Chemical

Hintergrund:

Synonyms: Vitamin D3 receptor,VDR,1,25-dihydroxyvitamin D3 receptor,Nuclear receptor subfamily 1 group I member 1,VDR,NR1I1,

Tissue Specificity: Heart, liver and skeletal muscle.

Background: VDR (Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al. (1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al. (2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB (TFIIB). A natural polymorphic variant of VDR, termed F/M4 (missing a FokI restriction site), which lacks only the first 3 amino acids (including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length (f/M1) receptor. Shah et al. (2006) stated that the signaling and oncogenic activity of beta-catenin (CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25- dihydroxyvitamin D3.

Sequence Similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.

Molekulargewicht: 48 kDa

Gen-ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway