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ULK3 Antikörper (C-Term)

ULK3 Reaktivität: Human, Maus, Ratte WB, IHC, ICC, FACS, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043954
  • Target Alle ULK3 Antikörper anzeigen
    ULK3 (Unc-51-Like Kinase 3 (ULK3))
    Bindungsspezifität
    • 9
    • 8
    • 8
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 427-454, C-Term
    Reaktivität
    • 42
    • 41
    • 17
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 45
    • 7
    • 2
    Kaninchen
    Klonalität
    • 47
    • 7
    Polyklonal
    Konjugat
    • 31
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ULK3 Antikörper ist unkonjugiert
    Applikation
    • 46
    • 30
    • 12
    • 5
    • 4
    • 3
    • 3
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Verwendungszweck
    Anti-ULK3 Antibody Picoband®
    Sequenz
    HTEVQNLMAR AEYLKEQVKM RESRWEAD
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-ULK3 Antibody Picoband® (ABIN3043954). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at C-terminus of human ULK3, different from the related mouse and rat sequences by four amino acids.
    Isotyp
    IgG
    Top Product
    Discover our top product ULK3 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry, 0.5-1 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Maloverjan A, Piirsoo M, Michelson P, Kogerman P, Osterlund T. ""Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway"". Exp Cell Res, 2010 Feb 15. 
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    ULK3 (Unc-51-Like Kinase 3 (ULK3))
    Andere Bezeichnung
    ULK3 (ULK3 Produkte)
    Hintergrund

    Synonyms: Serine/threonine-protein kinase ULK3,2.7.11.1,Unc-51-like kinase 3,ULK3,

    Tissue Specificity: Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord. .

    Background: Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. In search of potential homologues to Drosophila and human Fu, people have cloned human serine/threonine kinase ULK3 and assessed its ability to regulate GLI transcription factors, mediators of SHH signaling. And ULK3 enhances endogenous and over-expressed GLI1 and GLI2 transcriptional activity in cultured cells, as assessed by GLI-luciferase reporter assay. Besides that, ULK3 alters subcellular localization of GLI1, as assessed by immunofluorescent staining and immunoblotting assays. It is showed that ULK3 is an autophosphorylated kinase and phosphorylates GLI proteins in vitro. Also, ULK3 catalytical activity is crucial for its function in SHH pathway. It is demonstrated that ULK3 is widely expressed and its expression is higher in a number of tissues where Shh signaling is known to be active. The data suggest that serine/threonine kinase ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.

    Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

    Molekulargewicht
    53 kDa
    Gen-ID
    25989
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