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TFAM Antikörper (N-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TFAM in WB, IHC, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.
Produktnummer ABIN3043945

Kurzübersicht für TFAM Antikörper (N-Term) (ABIN3043945)

Target

Alle TFAM Antikörper anzeigen
TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reaktivität

  • 60
  • 33
  • 29
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 95
  • 6
Kaninchen

Klonalität

  • 85
  • 16
Polyklonal

Konjugat

  • 53
  • 10
  • 10
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser TFAM Antikörper ist unkonjugiert

Applikation

  • 81
  • 48
  • 27
  • 27
  • 25
  • 18
  • 16
  • 14
  • 14
  • 12
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 15
    • 9
    • 9
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 214-241, N-Term

    Verwendungszweck

    Anti-mtTFA/TFAM Antibody Picoband®

    Sequenz

    EMKSWEEQMI EVGRKDLLRR TIKKQRKY

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-mtTFA/TFAM Antibody Picoband® (ABIN3043945). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA, different from the related mouse and rat sequences by five amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    1. Larsson, N.-G., Oldfors, A., Garman, J. D., Barsh, G. S., Clayton, D. A. Down-regulation of mitochondrial transcription factor A during spermatogenesis in humans. Hum. Molec. Genet. 6: 185-191, 1997. 2. Milatovich, A., Parisi, M. A., Poulton, J., Clayton, D. A., Francke, U. Sequences homologous to MTTF1, mitochondrial transcription factor 1, are located on human chromosomes 7 (7pter-cen), 10 and 11 (11cen-qter). (Abstract) Cytogenet. Cell Genet. 58: 1924 only, 1992. 3. Scott, A. F. Personal Communication. Baltimore, Md. 9/20/2007.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Wu, Zhao, Xiao, Peng, Chen, He: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).

    Mo, Peng, Wang, Peng, Lan, Yu: "Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer." in: Oncology letters, Vol. 6, Issue 2, pp. 617-623, (2013) (PubMed).

  • Target

    TFAM (Transcription Factor A, Mitochondrial (TFAM))

    Andere Bezeichnung

    TFAM

    Hintergrund

    Synonyms: Transcription factor A, mitochondrial,mtTFA,Mitochondrial transcription factor 1,MtTF1,Transcription factor 6,TCF-6,Transcription factor 6-like 2,TFAM,TCF6, TCF6L2,

    Tissue Specificity: Ubiquitously expressed. .

    Background: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

    Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

    Molekulargewicht

    24 kDa

    Gen-ID

    7019

    UniProt

    Q00059

    Pathways

    Chromatin Binding
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