SOD2 Antikörper (C-Term)

Produktnummer ABIN3043935

Dieses Anti-SOD2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SOD2 in WB, IHC und ICC. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für SOD2 Antikörper (C-Term) (ABIN3043935)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-SOD2 Antikörper

Bindungsspezifität: AA 192-222, C-Term

Verwendungszweck: Anti-SOD2 Antibody Picoband®

Sequenz: QYKNVRPDYL KAIWNVINWE NVTERYMACK K

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SOD2 Antibody Picoband® (ABIN3043935). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SOD2, different from the related mouse sequence by one amino acid, and from the related rat sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Western blot, 0.1-0.5 μg/mL
1. Bastaki, M., Huen, K., Manzanillo, P., Chande, N., Chen, C., Balmes, J. R., Tager, I. B., Holland, N. Genotype-activity relationship for Mn-superoxide dismutase, glutathione peroxidase 1 and catalase in humans. Pharmacogenet. Genomics 16: 279-286, 2006. 2. Creagan, R., Tischfield, J., Ricciuti, F., Ruddle, F. H. Chromosome assignments of genes in man using mouse-human somatic cell hybrids: mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6. Humangenetik 20: 203-209, 1973. 3. Hiroi, S., Harada, H., Nishi, H., Satoh, M., Nagai, R., Kimura, A. Polymorphisms in the SOD2 and HLA-DRB1 genes are associated with nonfamilial idiopathic dilated cardiomyopathy in Japanese. Biochem. Biophys. Res. Commun. 261: 332-339, 1999.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-SOD2 Antikörper (ABIN3043935)

Zhang, Deng, Lai, Guan, Sun, Han, Wang, Pan, Ji, Luo, Huang, Tang, Gu, Dan, Yu, Namaka, Zhang, Deng, Li: "Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation." in: Scientific reports, Vol. 6, pp. 30146, (2018) (PubMed).

Details zu SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Andere Bezeichnung: SOD2

Hintergrund:

Synonyms: Superoxide dismutase [Mn], mitochondrial,1.15.1.1,SOD2,

Tissue Specificity: Expressed in brain and lymphoblasts. .

Background: SOD2 (Superoxide Dismutase 2), also called IPO-B or MNSOD, is a mitochondrial matrix enzyme that scavenges oxygen radicals produced by the extensive oxidation-reduction and electron transport reactions occurring in mitochondria. This gene is a member of the iron/manganese superoxide dismutase family. Using a somatic cell hybrid panel containing different segments of chromosome 6, they demonstrated that SOD2 is located in the region 6q25.3-qter which, together with the FISH analysis, indicated that SOD2 is in the distal portion of 6q25. The SOD2 gene encodes an intramitochondrial free radical scavenging enzyme that is the first line of defense against superoxide produced as a byproduct of oxidative phosphorylation. Adeno-associated viral delivery of the human SOD2 gene resulted in suppression of optic nerve degeneration and rescue of retinal ganglion cells. The findings suggested that reactive oxygen species contributed to retinal cell death and optic nerve damage in mice with complex I deficiency, and that expression of SOD2 attenuated the disease process.

Sequence Similarities: Belongs to the iron/manganese superoxide dismutase family.

Molekulargewicht: 24 kDa

Gen-ID: 6648

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling