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PKC iota Antikörper (AA 21-214)

PRKCI Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043909
  • Target Alle PKC iota (PRKCI) Antikörper anzeigen
    PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
    Bindungsspezifität
    • 12
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 21-214
    Reaktivität
    • 31
    • 24
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 25
    • 6
    Kaninchen
    Klonalität
    • 25
    • 6
    Polyklonal
    Konjugat
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PKC iota Antikörper ist unkonjugiert
    Applikation
    • 30
    • 12
    • 12
    • 12
    • 11
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Verwendungszweck
    Anti-PKC iota/PRKCI Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-PKC iota/PRKCI Antibody Picoband® (ABIN3043909). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human PKC iota recombinant protein (Position: D21-Q214). Human PKC iota shares 96% and 97% amino acid (aa) sequence identity with mouse and rat PKC iota, respectively.
    Isotyp
    IgG
    Top Product
    Discover our top product PRKCI Primärantikörper
  • Applikationshinweise
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    1. Atwood, S. X., Li, M., Lee, A., Tang, J. Y., Oro, A. E. GLI activation by atypical protein kinase C lambda/iota regulates the growth of basal cell carcinomas. Nature 494: 484-488, 2013. 2. De Donato, M., Gallagher Jr., D. S., Davis, S. K, Stelly, D. M., Taylor, J.F. The assignment ofPRKCI to bovine chromosome 1q34-q36 by FISH suggests a new assignment to human chromosome 3. Cytogenet. Cell Genet. 95: 79-81, 2001. 3. Mazzarella R, Ciccodicola A, Esposito T, Arcucci A, Migliaccio C, Jones C, Schlessinger D, D'Urso M, D'Esposito M (August 1995). "Human protein kinase C Iota gene (PRKCI) is closely linked to the BTK gene in Xq21.3". Genomics 26(3): 629-31.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
    Andere Bezeichnung
    PRKCI (PRKCI Produkte)
    Hintergrund

    Synonyms: Protein kinase C iota type,2.7.11.13,Atypical protein kinase C-lambda/iota,PRKC-lambda/iota,aPKC-lambda/iota,nPKC-iota,PRKCI,DXS1179E,

    Tissue Specificity: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers. .

    Background: Protein kinase C iota type, also called PKCI, is an enzyme that in humans is encoded by the PRKCI gene. This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. PRKCI is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKCI is also a hedgehog target gene that forms a positive feedback loop with GLI and exists at increased levels in basal cell carcinomas.

    Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

    Molekulargewicht
    68 kDa
    Gen-ID
    5584
    UniProt
    P41743
    Pathways
    Neurotrophin Signalübertragung, Cell-Cell Junction Organization, Tube Formation
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