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PPP1R12A Antikörper (N-Term)

Dieses Anti-PPP1R12A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PPP1R12A in WB, IHC, IF, FACS und ICC. Geeignet für Human, Maus, Ratte und Affe. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Produktnummer ABIN3043902

Kurzübersicht für PPP1R12A Antikörper (N-Term) (ABIN3043902)

Target

Alle PPP1R12A Antikörper anzeigen
PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reaktivität

  • 69
  • 60
  • 44
  • 6
  • 6
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
Human, Maus, Ratte, Affe

Wirt

  • 92
  • 1
  • 1
Kaninchen

Klonalität

  • 92
  • 2
Polyklonal

Konjugat

  • 53
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser PPP1R12A Antikörper ist unkonjugiert

Applikation

  • 60
  • 26
  • 26
  • 26
  • 22
  • 11
  • 9
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 26
    • 16
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-40, N-Term

    Verwendungszweck

    Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband®

    Sequenz

    MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband® (ABIN3043902). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human PPP1R12A, identical to the related mouse and rat sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Kimura, K., Ito, M., Amano, M., Chihara, K., Fukata, Y., Nakafuku, M., Yamamori, B., Feng, J., Nakano, T., Okawa, K., Iwamatsu, A., Kaibuchi, K. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245-248, 1996. 2. Takahashi, N., Ito, M., Tanaka, J., Nakano, T., Kaibuchi, K., Odai, H., Takemura, K. Localization of the gene coding for myosin phosphatase, target subunit 1 (MYPT1) to human chromosome 12q15-q21. Genomics 44: 150-152, 1997.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Yan, Wang, Jiang, Chen, He, Mang, Shao, Xu: "The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia." in: Neural regeneration research, Vol. 10, Issue 9, pp. 1441-9, (2015) (PubMed).

  • Target

    PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

    Andere Bezeichnung

    PPP1R12A

    Hintergrund

    Synonyms: Protein phosphatase 1 regulatory subunit 12A,Myosin phosphatase-targeting subunit 1,Myosin phosphatase target subunit 1,Protein phosphatase myosin-binding subunit,PPP1R12A,MBS, MYPT1,

    Tissue Specificity: Expressed in striated muscles, specifically in type 2a fibers (at protein level). .

    Background: PPP1R12A (Protein phosphatase 1 regulatory subunit 12A), also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in two ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

    Molekulargewicht

    130 kDa

    Gen-ID

    4659

    UniProt

    O14974

    Pathways

    M Phase
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