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CD3 epsilon Antikörper (AA 23-207)

CD3E Reaktivität: Human, Maus, Ratte, Huhn WB, IHC, IF, IHC (fro), ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043805
  • Target Alle CD3 epsilon (CD3E) Antikörper anzeigen
    CD3 epsilon (CD3E)
    Bindungsspezifität
    • 24
    • 20
    • 15
    • 15
    • 12
    • 9
    • 8
    • 8
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 23-207
    Reaktivität
    • 226
    • 173
    • 50
    • 40
    • 18
    • 8
    • 8
    • 8
    • 7
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Huhn
    Wirt
    • 131
    • 127
    • 47
    • 22
    • 21
    • 10
    • 1
    • 1
    • 1
    • 1
    Kaninchen
    Klonalität
    • 250
    • 104
    • 7
    Polyklonal
    Konjugat
    • 137
    • 32
    • 30
    • 22
    • 18
    • 8
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD3 epsilon Antikörper ist unkonjugiert
    Applikation
    • 266
    • 139
    • 101
    • 82
    • 80
    • 70
    • 28
    • 25
    • 22
    • 20
    • 15
    • 13
    • 13
    • 12
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Verwendungszweck
    Anti-CD3 epsilon/CD3E Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-CD3 epsilon/CD3E Antibody Picoband® (ABIN3043805). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
    Isotyp
    IgG
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat, Chicken
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
    Immunocytochemistry , 0.5-1 μg/mL, Human
    Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
    1. Clevers HC, Dunlap S, Wileman TE, Terhorst C (November 1988)."Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter". Proc. Natl. Acad. Sci. U.S.A. 85(21): 8156-60. 2. "Entrez Gene: CD3E CD3e molecule, epsilon (CD3-TCR complex)" 3. Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C (1986). "Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex". Nature 321 (6068): 431-4.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Guo, Qian, Zhang: "The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis." in: BMC ophthalmology, Vol. 16, pp. 66, (2017) (PubMed).

    Chen, Liu, Xu, Yang, Zhang, Zhang: "Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 1063-1067, (2015) (PubMed).

    Huang, Zhu, Zhang, Zhu, Liu, Zhu, Wang, Li, Yang, Dong, Liu, Chen, Zhang, Yang, Deng, Fan, Wang, Liu, Ma, Fu, Wu: "S100+ cells: a new neuro-immune cross-talkers in lymph organs." in: Scientific reports, Vol. 3, pp. 1114, (2013) (PubMed).

    Li, Geng, Yin, Chen, Yang, Wu, Li, Du, Schmidt, Przybylski: "Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients." in: Journal of translational medicine, Vol. 8, pp. 47, (2010) (PubMed).

    Xie, Jia, He, Gao: "Morphological observation of tumor infiltrating immunocytes in human rectal cancer." in: World journal of gastroenterology, Vol. 12, Issue 11, pp. 1757-60, (2006) (PubMed).

    Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

  • Target
    CD3 epsilon (CD3E)
    Andere Bezeichnung
    CD3E (CD3E Produkte)
    Hintergrund

    Synonyms: T-cell surface glycoprotein CD3 epsilon chain,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E,

    Tissue Specificity: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. .

    Background: CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

    Sequence Similarities: Contains 1 Ig-like (immunoglobulin-like) domain.

    Molekulargewicht
    23 kDa
    Gen-ID
    916
    UniProt
    P07766
    Pathways
    T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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