BDNF Antikörper (AA 129-247)

Produktnummer ABIN3043798

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BDNF in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 10+ Publikationen erwähnt.

Kurzübersicht für BDNF Antikörper (AA 129-247) (ABIN3043798)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BDNF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-BDNF Antikörper

Bindungsspezifität: AA 129-247

Verwendungszweck: Anti-BDNF Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-BDNF Antibody Picoband® (ABIN3043798). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat1. Jones, K. R., Reichardt, L. F. : Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Nat. Acad. Sci. 87: 8060-8064, 1990. 2. Lee, R., Kermani, P., Teng, K. K., Hempstead, B. L. : Regulation of cell survival by secreted proneurotrophins. Science 294: 1945-1948, 2001 . 3. Pruunsild, P., Kazantseva, A., Aid, T., Palm, K., Timmusk, T. : Dissecting the human BDNF locus: biional transcription, complex splicing, and multiple promoters. Genomics 90: 397-406, 2007.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-BDNF Antikörper (ABIN3043798)

Yin, Liang, Wang, Yan, Yin, Wu, Su: "Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 649-658, (2018) (PubMed).

Li, Wu, Miao, Xiong et al.: "Effects of Polysaccharide of Gastrodia Elata Blume and Electro-Acupuncture on Expressions of Brain-Derived Neurotrophic Factor and Stem Cell Factor Protein in Caudate Putamen of Focal Cerebral ..." in: Medical science monitor basic research, Vol. 22, pp. 175-180, (2017) (PubMed).

Zheng, Lu, Guo, Zhang, Liu, Guo, Huang, Han: "Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 7893710, (2016) (PubMed).

Tong, Wang, Li, Zhu, Chen, Hua: "Effects of senescence on the expression of BDNF and TrkB receptor in the lateral geniculate nucleus of cats." in: Dong wu xue yan jiu = Zoological research, Vol. 36, Issue 1, pp. 48-53, (2015) (PubMed).

Zhao, Chen, Shen, Zhao, Wang, Shi, Wang, Cui, Yan, Xue: "Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury." in: Neural regeneration research, Vol. 7, Issue 10, pp. 741-8, (2015) (PubMed).

Tan, Shi, Shi, Liu, Liu, Wang, Chen, Xing, Shen, Jia, Ye, He, Li: "Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles." in: Neural regeneration research, Vol. 8, Issue 3, pp. 233-43, (2014) (PubMed).

Li, Li, Li, Quan, Wang: "Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease." in: Neural regeneration research, Vol. 7, Issue 36, pp. 2860-6, (2014) (PubMed).

Wang, Feng, Fu, Li: "Panax notoginsenoside Rb1 ameliorates Alzheimer's disease by upregulating brain-derived neurotrophic factor and downregulating Tau protein expression." in: Experimental and therapeutic medicine, Vol. 6, Issue 3, pp. 826-830, (2013) (PubMed).

Li, Xia, Zhang, Wu: "S100B protein, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in human milk." in: PLoS ONE, Vol. 6, Issue 6, pp. e21663, (2011) (PubMed).

Shi, Shao, Yuan, Pan, Li: "Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus." in: Yonsei medical journal, Vol. 51, Issue 5, pp. 661-71, (2010) (PubMed).

Details zu BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF

Hintergrund:

Synonyms: Brain-derived neurotrophic factor,BDNF,Bdnf,

Background: Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Molekulargewicht: 14 kDa

Gen-ID: 12064

UniProt: P21237

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity