C5 Antikörper (AA 1-77)

Produktnummer ABIN3043765

Produktdetails anti-C5 Antikörper

Target: C5 (Complement Component 5 (C5))

Bindungsspezifität: AA 1-77

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser C5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Rat.
Gene Name: complement component 5
Protein Name: Complement C5

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived rat C5a recombinant protein (Position: D1-R77). Rat C5a shares 62% and 82% amino acid (aa) sequences identity with human and mouse C5a, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, The detection limit for C5a is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu C5

Target: C5 (Complement Component 5 (C5))

Andere Bezeichnung: C5 (C5 Produkte)

Hintergrund: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. C5a plays a key role in increasing migration and adherence of neutrophils and monocytes to vessel walls. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Synonyms: Anaphylatoxin C5a analog antibody|Anaphylatoxin C5a antibody|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody|C5 antibody|C5a antibody|CO5_HUMAN antibody|Complement C5 alpha'' chain antibody|Complement C5 antibody|Complement component C5 antibody|Complement component C5a antibody|CPAMD 4 antibody|CPAMD4 antibody

Gen-ID: 362119

UniProt: P08650

Pathways: Komplementsystem, Carbohydrate Homeostasis