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BUB1 Antikörper (AA 731-1085)

BUB1 Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3043764
  • Target Alle BUB1 Antikörper anzeigen
    BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
    Bindungsspezifität
    • 15
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 731-1085
    Reaktivität
    • 73
    • 30
    • 9
    • 1
    Human, Maus, Ratte
    Wirt
    • 65
    • 10
    Kaninchen
    Klonalität
    • 63
    • 12
    Polyklonal
    Konjugat
    • 46
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser BUB1 Antikörper ist unkonjugiert
    Applikation
    • 58
    • 36
    • 19
    • 14
    • 13
    • 13
    • 10
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Verwendungszweck
    Anti-Bub1 AAMP Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-Bub1 AAMP Antibody Picoband® (ABIN3043764). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.
    Isotyp
    IgG
    Top Product
    Discover our top product BUB1 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    1. "Entrez Gene: BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast)" 2. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B (March 1998). "Mutations of mitotic checkpoint genes in human cancers". Nature 392 (6673): 300-3. 3. Roberts BT, Farr KA, Hoyt MA (December 1994). "The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase". Mol. Cell. Biol. 14 (12): 8282-91.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
    Andere Bezeichnung
    BUB1 (BUB1 Produkte)
    Hintergrund

    Synonyms: Angio-associated migratory cell protein,AAMP,

    Tissue Specificity: Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics. .

    Background: BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

    Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

    Molekulargewicht
    130 kDa
    Gen-ID
    699
    UniProt
    Q13685
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