alpha Fetoprotein Antikörper (AA 378-609)

Produktnummer ABIN3043730

Der Kaninchen Polyklonal Anti-alpha Fetoprotein-Antikörper wurde für WB und IHC validiert. Er ist geeignet, alpha Fetoprotein in Proben von Human zu detektieren. Es sind 11+ Publikationen verfügbar.

Kurzübersicht für alpha Fetoprotein Antikörper (AA 378-609) (ABIN3043730)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-alpha Fetoprotein Antikörper

Bindungsspezifität: AA 378-609

Verwendungszweck: Anti-alpha 1 Fetoprotein/AFP Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-alpha 1 Fetoprotein/AFP Antibody Picoband® (ABIN3043730). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human AFP recombinant protein (Position: Q378-V609). Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
1. Carter CS (2002). "Neuroendocrinology of sexual behavior in the female". In Becker JB. Behavioral Endocrinology. Cambridge, Mass: MIT Press. pp.88-89. ISBN 0-262-52321-3 2. Bader D, Riskin A, Vafsi O, Tamir A, Peskin B, Israel N, Merksamer R, Dar H, David M (November 2004). "Alpha-fetoprotein in the early neonatal period--a large study and review of the literature". Clin. Chim. Acta 349 (1-2): 15-23. 3. Blair JI, Carachi R, Gupta R, Sim FG, McAllister EJ, Weston R (April 1987). "Plasma alpha fetoprotein reference ranges in infancy: effect of prematurity". Arch. Dis. Child. 62 (4): 362-9.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-alpha Fetoprotein Antikörper (ABIN3043730)

Jeng, Jeng, Jeng, Sheen, Li, Lu, Chang: "Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells." in: American journal of surgery, Vol. 215, Issue 4, pp. 735-743, (2017) (PubMed).

Winkler, Hempel, Brückner, Mallek, Weise, Liehr, Tautenhahn, Bartels, Christ: "Mouse white adipose tissue-derived mesenchymal stem cells gain pericentral and periportal hepatocyte features after differentiation in vitro, which are preserved in vivo after hepatic transplantation." in: Acta physiologica (Oxford, England), Vol. 215, Issue 2, pp. 89-104, (2016) (PubMed).

Li, Wu, Liu: "An immune sandwich assay of carcinoembryonic antigen based on the joint use of upconversion phosphors and magnetic beads." in: The Analyst, Vol. 140, Issue 12, pp. 4083-8, (2015) (PubMed).

Yan, Zhu, Sun, Zhang, Li, Sun, Li, Qian, Zhu, Xu: "Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 4, pp. 534-45, (2015) (PubMed).

Wang, Xu, Zhou, Zhong, Wen, Yu, Chen, Shen, Chen, She, Jiang, Miao, Wei: "The viral oncoprotein HBx of Hepatitis B virus promotes the growth of hepatocellular carcinoma through cooperating with the cellular oncoprotein RMP." in: International journal of biological sciences, Vol. 10, Issue 10, pp. 1181-92, (2014) (PubMed).

Shan, Wu, Li, Shen, Wang, Liu, Shen, Lei: "Continuous passages accelerate the reprogramming of mouse induced pluripotent stem cells." in: Cellular reprogramming, Vol. 16, Issue 1, pp. 77-83, (2014) (PubMed).

Yin, Zhu, Yang, Ni, Zhou, Chen, Wen: "Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cells in vivo and in vitro." in: Molecular medicine reports, Vol. 11, Issue 3, pp. 1722-32, (2014) (PubMed).

Wu, Gu, Zhao, Tan, Chen, Zhu, Zhang: "Derivation of multipotent nestin(+)/CD271 (-)/STRO-1 (-) mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions." in: Human cell, Vol. 26, Issue 1, pp. 19-27, (2013) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Qin, Hua, Shuang, Wang, Cui: "Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro." in: Pharmaceutical biology, Vol. 48, Issue 3, pp. 275-81, (2010) (PubMed).

Su, Wu, Tan, Li, Chen, Luo, Zhang: "Early homing behavior of Stro-1- mesenchyme-like cells derived from human embryonic stem cells in an immunocompetent xenogeneic animal model." in: Biochemical and biophysical research communications, Vol. 394, Issue 3, pp. 616-22, (2010) (PubMed).

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: AFP

Hintergrund:

Synonyms: Alpha-fetoprotein,Alpha-1-fetoprotein,Alpha-fetoglobulin,AFP,HPAFP,

Tissue Specificity: Plasma. Synthesized by the fetal liver and yolk sac.

Background: AFP, also called Alpha-fetoprotein, alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.

Sequence Similarities: Belongs to the ALB/AFP/VDB family.

Molekulargewicht: 69 kDa

Gen-ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process