Lamin A/C Antikörper (C-Term)

Produktnummer ABIN3043695

Dieses Anti-Lamin A/C-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Lamin A/C in WB, IHC und ICC. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Lamin A/C Antikörper (C-Term) (ABIN3043695)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-Lamin A/C Antikörper

Bindungsspezifität: AA 455-469, C-Term

Verwendungszweck: Anti-Lamin A + C/LMNA Antibody Picoband®

Sequenz: RNKSNEDQSM GNWQI

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Lamin A + C/LMNA Antibody (ABIN3043695). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Lammerding, J., Schulze, P. C., Takahashi, T., Kozlov, S., Sullivan, T., Kamm, R. D., Stewart, C. L., Lee, R. T. : Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113: 370-378, 2004. 2. Lin, F., Worman, H. J. : Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268: 16321-16326, 1993. 3. Wydner, K. L., McNeil, J. A., Lin, F., Worman, H. J., Lawrence, J. B. : Chromosomal assignment of human nuclear envelope protein genes LMNA, LMNB1, and LBR by fluorescence in situ hybridization. Genomics 32: 474-478, 1996.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Lamin A/C Antikörper (ABIN3043695)

Jevti?, Edens, Li, Nguyen, Chen, Levy: "Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells." in: The Journal of biological chemistry, Vol. 290, Issue 46, pp. 27557-71, (2015) (PubMed).

Liu, Ma, Wu, Li, Zhang, Han, Wang, Reindl, Wu, Ma: "Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-?B signaling." in: BMC cancer, Vol. 14, pp. 686, (2014) (PubMed).

Yang, Liu, Zhu, Zhang, Xi, Hu, He, Jiang: "Human peripheral nerve-derived scaffold for tissue-engineered nerve grafts: histology and biocompatibility analysis." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 96, Issue 1, pp. 25-33, (2010) (PubMed).

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: LMNA

Hintergrund:

Synonyms: Prelamin-A/C,Lamin-A/C,70 kDa lamin,Renal carcinoma antigen NY-REN-32,LMNA,LMN1,

Tissue Specificity: In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress. .

Background: Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Sequence Similarities: Belongs to the intermediate filament family.

Molekulargewicht: 70-74 kDa

UniProt: P02545

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus