PKLR Antikörper (C-Term)

Produktnummer ABIN3043607

Der Kaninchen Polyklonal Anti-PKLR-Antikörper wurde für WB und IHC validiert. Er ist geeignet, PKLR in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PKLR Antikörper (C-Term) (ABIN3043607)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKLR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-PKLR Antikörper

Bindungsspezifität: AA 522-552, C-Term

Verwendungszweck: Anti-PKLR Antibody Picoband®

Sequenz: EAIWADDVDR RVQFGIESGK LRGFLRVGDL V

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-PKLR Antibody Picoband® (ABIN3043607). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: PKLR pyruvate kinase, liver and RBC". 2. Tani K, Fujii H, Tsutsumi H, Sukegawa J, Toyoshima K, Yoshida MC, Noguchi T, Tanaka T, Miwa S (Apr 1987). "Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment". Biochem Biophys Res Commun 143 (2): 431-8.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Andere Bezeichnung: PKLR

Hintergrund:

Synonyms: Pyruvate kinase PKLR,2.7.1.40,Pyruvate kinase 1,Pyruvate kinase isozymes L/R,R-type/L-type pyruvate kinase,Red cell/liver pyruvate kinase,PKLR,PK1, PKL,

Tissue Specificity: Ubiquitous, expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.

Background: Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the pyruvate kinase family.

Molekulargewicht: 62 kDa

Gen-ID: 5313

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process