PGK1 Antikörper (C-Term)

Produktnummer ABIN3043604

Der Kaninchen Polyklonal Anti-PGK1-Antikörper wurde für WB, IF, FACS und ICC validiert. Er ist geeignet, PGK1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für PGK1 Antikörper (C-Term) (ABIN3043604)

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PGK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-PGK1 Antikörper

Bindungsspezifität: AA 312-337, C-Term

Verwendungszweck: Anti-PGK1 Antibody Picoband®

Sequenz: MGLDCGPESS KKYAEAVTRA KQIVWN

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PGK1 Antibody Picoband® (ABIN3043604). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PGK1, different from the related mouse and rat sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: PGK1 phosphoglycerate kinase 1". 2. Michelson AM, Markham AF, Orkin SH (May 1983). "Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase". Proc Natl Acad Sci U S A 80 (2): 472-6. 3. Singer-Sam J, Keith DH, Tani K, Simmer RL, Shively L, Lindsay S, Yoshida A, Riggs AD (May 1985). "Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase]". Gene 32 (3): 409-17.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Andere Bezeichnung: PGK1

Hintergrund:

Synonyms: Phosphoglycerate kinase 1,2.7.2.3,Cell migration-inducing gene 10 protein,Primer recognition protein 2,PRP 2,PGK1,PGKA,MIG10, OK/SW-cl.110,

Tissue Specificity: Ubiquitous, highest levels of expression in heart and skeletal muscle. .

Background: PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Molekulargewicht: 43 kDa

Gen-ID: 5230

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process