TdT Antikörper (AA 316-509)

Produktnummer ABIN3043577

Produktdetails anti-TdT Antikörper

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Bindungsspezifität: AA 316-509

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TdT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-TdT/DNTT Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-TdT/DNTT Antibody Picoband® (ABIN3043577). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. Landau, N. R., St. John, T. P., Weissman, I. L., Wolf, S. C., Silverstone, A. E., Baltimore, D. : Cloning of terminal transferase cDNA by antibody screening. Proc. Nat. Acad. Sci. 81: 5836-5840, 1984. 2. Yang-Feng, T. L., Landau, N. R., Baltimore, D., Francke, U.: The terminal deoxynucleotidyltransferase gene is located on human chromosome 10 (10q23-q24) and on mouse chromosome 19. Cytogenet. Cell Genet. 43: 121-126, 1986. 3. Riley, L. K., Morrow, J. K., Danton, M. J., Coleman, M. S.: Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5-prime flanking region. Proc. Nat. Acad. Sci. 85: 2489-2493, 1988.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-TdT Antikörper (ABIN3043577)

Yang, Zhang, Ding, Huang, Chen, Wu, Wang, Wang, Chen: "Antitumour activity of the recombination polypeptide GST-NT21MP is mediated by inhibition of CXCR4 pathway in breast cancer." in: British journal of cancer, Vol. 110, Issue 5, pp. 1288-97, (2014) (PubMed).

Zhang, Pei, Ru, Li, Liu: "Bone morphogenetic protein 7 upregulates the expression of nestin and glial fibrillary acidic protein in rats with cerebral ischemia-reperfusion injury." in: Biomedical reports, Vol. 1, Issue 6, pp. 895-900, (2014) (PubMed).

Tochinai, Ando, Suzuki, Suzuki, Nagata, Hata, Uchida, Kobayashi, Kado, Kaneko: "Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft fu?r Toxikologische Pathologie, Vol. 65, Issue 6, pp. 737-43, (2013) (PubMed).

Zeng, Ke, Song, Yao, Hu, Zhang, Li, Yin: "Annexin A3 is associated with a poor prognosis in breast cancer and participates in the modulation of apoptosis in vitro by affecting the Bcl-2/Bax balance." in: Experimental and molecular pathology, Vol. 95, Issue 1, pp. 23-31, (2013) (PubMed).

Xi, Wang, Ye, Luo, Ning: "Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice." in: PLoS ONE, Vol. 8, Issue 11, pp. e82330, (2013) (PubMed).

Gong, Zheng, Wang, Chen, Pang, Hu, Cao: "Coexistence of hyperlipidemia and acute cerebral ischemia/reperfusion induces severe liver damage in a rat model." in: World journal of gastroenterology, Vol. 18, Issue 35, pp. 4934-43, (2012) (PubMed).

Luo, Xia: "A small dose of hydrogen peroxide enhances tumor necrosis factor-alpha toxicity in inducing human vascular endothelial cell apoptosis: reversal with propofol." in: Anesthesia and analgesia, Vol. 103, Issue 1, pp. 110-6, table of contents, (2006) (PubMed).

Luo, Xia, Ansley, Ouyang, Granville, Li, Xia, Zhou, Liu: "Propofol dose-dependently reduces tumor necrosis factor-alpha-Induced human umbilical vein endothelial cell apoptosis: effects on Bcl-2 and Bax expression and nitric oxide generation." in: Anesthesia and analgesia, Vol. 100, Issue 6, pp. 1653-9, (2005) (PubMed).

Xu, Liu, Gao, Zhang, Wang, Cao: "Possible mechanisms of the protection of ginsenoside Re against MPTP-induced apoptosis in substantia nigra neurons of Parkinson's disease mouse model." in: Journal of Asian natural products research, Vol. 7, Issue 3, pp. 215-24, (2004) (PubMed).

Details zu TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Andere Bezeichnung: DNTT (DNTT Produkte)

Hintergrund:

Synonyms: DNA nucleotidylexotransferase,2.7.7.31 ,Terminal addition enzyme,Terminal deoxynucleotidyltransferase,Terminal transferase ,DNTT,TDT ,

Tissue Specificity: Widely expressed. .

Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Sequence Similarities: Belongs to the DNA polymerase type-X family.

Molekulargewicht: 58 kDa

Gen-ID: 1791

UniProt: P04053

Pathways: DNA Reparatur