NK2 Homeobox 5 Antikörper (Middle Region)

Produktnummer ABIN3043568

Der Kaninchen Polyklonal Anti-NK2 Homeobox 5-Antikörper wurde für WB validiert. Er ist geeignet, NK2 Homeobox 5 in Proben von Human und Maus zu detektieren.

Kurzübersicht für NK2 Homeobox 5 Antikörper (Middle Region) (ABIN3043568)

Target: NK2 Homeobox 5 (NKX2-5)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NK2 Homeobox 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-NK2 Homeobox 5 Antikörper

Bindungsspezifität: AA 103-132, Middle Region

Verwendungszweck: Anti-Nkx2.5/NKX2-5 Antibody Picoband®

Sequenz: AKDPRAEKKE LCALQKAVEL EKTEADNAER

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Nkx2.5/NKX2-5 Antibody Picoband® (ABIN3043568). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5, different from the related mouse and rat sequences by five amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Hiroi, Y., Kudoh, S., Monzen, K., Ikeda, Y., Yazaki, Y., Nagai, R., Komuro, I. Tbx5 associates withNkx2-5 and synergistically promotes cardiomyocyte differentiation. Nature Genet. 28: 276-280, 2001. 2. Shiojima, I., Komuro, I., Inazawa, J., Nakahori, Y., Matsushita, I., Abe, T., Nagai, R., Yazaki, Y.Assignment of cardiac homeobox gene CSX to human chromosome 5q34. Genomics 27: 204-206, 1995. 3. Turbay D, Wechsler SB, Blanchard KM, Izumo S (January 1997). "Molecular cloning, chromosomal mapping, and characterization of the human cardiac-specific homeobox gene hCsx". Mol Med 2 (1): 86-96.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NK2 Homeobox 5

Target: NK2 Homeobox 5 (NKX2-5)

Andere Bezeichnung: NKX2-5

Hintergrund:

Synonyms: Homeobox protein Nkx-2.5,Cardiac-specific homeobox,Homeobox protein CSX,Homeobox protein NK-2 homolog E,NKX2-5,CSX, NKX2.5, NKX2E,

Tissue Specificity: Expressed only in the heart.

Background: Homeobox protein Nkx-2.5, also known as NKX2E or CSX is a protein that in humans is encoded by the NKX2-5 gene. It is mapped to 5q35.1. Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. Nkx2.5 and Tbx5 ly bound to the promoter of the gene encoding cardiac-specific natriuretic peptide precursor type A (NPPA) in tandem, and both transcription factors showed synergistic activation. The cardiac homeobox protein Nkx2.5 is essential in cardiac development, and mutations in CSX (which encodes Nkx2.5) cause various congenital heart malformations.

Sequence Similarities: Belongs to the NK-2 homeobox family.

Molekulargewicht: 35 kDa

Gen-ID: 1482

UniProt: P52952

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development