CD8 alpha Antikörper (AA 22-187)

Produktnummer ABIN3043499

Produktdetails anti-CD8 alpha Antikörper

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Bindungsspezifität: AA 22-187

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD8 alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for T-cell surface glycoprotein CD8 alpha chain(CD8A) detection. Tested with WB, IHC-P in Human.
Gene Name: CD8a molecule
Protein Name: T-cell surface glycoprotein CD8 alpha chain

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187). Human CD8 alpha shares 49% and 47% amino acid (aa) sequence identity with mouse and rat CD8 alpha, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD8 alpha is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-CD8 alpha Antikörper (ABIN3043499)

Xu, Chen, Zhao, Huang, Duan, Tong, Zhang, Li, Chang, Chen: "The CD8? gene in duck (Anatidae): cloning, characterization, and expression during viral infection." in: Molecular biology reports, Vol. 42, Issue 2, pp. 431-9, (2015) (PubMed).

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, (2015) (PubMed).

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, (2005) (PubMed).

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8A (CD8A Produkte)

Synonyme: cd8 antikoerper, LOC100136450 antikoerper, BB154331 antikoerper, Ly-2 antikoerper, Ly-35 antikoerper, Ly-B antikoerper, Lyt-2 antikoerper, zgc:136643 antikoerper, CD8 antikoerper, Leu2 antikoerper, MAL antikoerper, p32 antikoerper, RHACD8-4 antikoerper, CD8a molecule antikoerper, uncharacterized LOC100125537 antikoerper, CD8 alpha antikoerper, CD8 antigen, alpha chain antikoerper, T-cell surface glycoprotein CD8 alpha chain antikoerper, Cd8a antikoerper, LOC100125537 antikoerper, LOC100136450 antikoerper, CD8A antikoerper, cd8a antikoerper, LOC100126556 antikoerper

Hintergrund: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Synonyms: alpha polypeptide (p32) antibody|CD 8a antibody|CD8 alpha chain antibody|CD8 antibody|CD8 antigen alpha chain antibody|CD8 antigen alpha polypeptide antibody|CD8 antigen alpha polypeptide (p32) antibody|CD8a antibody|CD8a antigen antibody|CD8a molecule antibody|CD8A_HUMAN antibody|Leu 2 antibody|Leu2 antibody|Leu2 T lymphocyte antigen antibody|Ly3 antibody|LYT3 antibody|MAL antibody|OKT8 T cell antigen antibody|OTTHUMP00000160760 antibody|OTTHUMP00000160764 antibody|OTTHUMP00000203528 antibody|OTTHUMP00000203721 antibody|p32 antibody|T cell antigen Leu2 antibody|T cell co receptor antibody|T cell coreceptor antibody|T cell surface glycoprotein CD8 alpha chain antibody|T lymphocyte differentiation antigen T8/Leu 2 antibody|T lymphocyte differentiation antigen T8/Leu2 antibody|T-cell surface glycoprotein CD8 alpha chain antibody|T-lymphocyte differentiation antigen T8/Leu-2 antibody|T8 T cell antigen antibody|T8/Leu-2 T-lymphocyte differentiation antigen antibody

Gen-ID: 925

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg