LIM Domain Kinase 1 Antikörper (C-Term)

Produktnummer ABIN3043411

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LIM Domain Kinase 1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für LIM Domain Kinase 1 Antikörper (C-Term) (ABIN3043411)

Target: LIM Domain Kinase 1 (LIMK1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LIM Domain Kinase 1 Antikörper

Bindungsspezifität: AA 599-634, C-Term

Verwendungszweck: Anti-LIMK1 Antibody Picoband®

Sequenz: KLEHWLETLR MHLAGHLPLG PQLEQLDRGF WETYRR

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-LIMK1 Antibody Picoband® (ABIN3043411). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human LIMK1 , different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: LIMK1 LIM domain kinase 1". 2. Osborne LR, Martindale D, Scherer SW, Shi XM, Huizenga J, Heng HH, Costa T, Pober B, Lew L, Brinkman J, Rommens J, Koop B, Tsui LC (Jan 1997). "Identification of genes from a 500-kb region at 7q11.23 that is commonly deleted in Williams syndrome patients". Genomics 36 (2): 328-36. 3. Tassabehji M, Metcalfe K, Fergusson WD, Carette MJ, Dore JK, Donnai D, Read AP, Pröschel C, Gutowski NJ, Mao X, Sheer D (Aug 1996). "LIM-kinase deleted in Williams syndrome". Nat. Genet. 13 (3): 272-3.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-LIM Domain Kinase 1 Antikörper (ABIN3043411)

Zhang, Gan, Zhou: "Immunohistochemical investigation of the correlation between LIM kinase 1 expression and development and progression of human ovarian carcinoma." in: The Journal of international medical research, Vol. 40, Issue 3, pp. 1067-73, (2013) (PubMed).

Details zu LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung: LIMK1

Hintergrund:

Synonyms: LIM domain kinase 1,LIMK-1,2.7.11.1,LIMK1,LIMK,

Tissue Specificity: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Background: LIM domain kinase 1?is an?enzyme?that in humans is encoded by the?LIMK1?gene. There are approximately 40 known eukaryotic LIM proteins, so named for the?LIM domains?they contain. LIM domains are highly conserved?cysteine-rich structures containing 2?zinc fingers. Although zinc fingers usually function by binding to?DNA?or?RNA, the LIM motif probably mediates?protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2?N-terminal?LIM motifs, a central?PDZ domain, and a?C-terminal?protein kinase?domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development.

Molekulargewicht: 72 kDa

Gen-ID: 3984

UniProt: P53667

Pathways: Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events