LIF Antikörper (Leukemia Inhibitory Factor) (AA 23-202)

Details for Product anti-LIF Antibody No. ABIN3043403, Anbieter: Anmelden zum Anzeigen
Antigen
  • CDF
  • DIA
  • HILDA
  • MLPLI
  • LIF, interleukin 6 family cytokine
  • leukemia inhibitory factor
  • LIF
  • Lif
Epitop
AA 23-202
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Reaktivität
Human
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Wirt
Kaninchen
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Klonalität
Polyklonal
Konjugat
Dieser LIF Antikörper ist unkonjugiert
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Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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12
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Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Rabbit IgG polyclonal antibody for Leukemia inhibitory factor(LIF) detection. Tested with WB, IHC-P in Human.
Immunogen E.coli-derived human LIF recombinant protein (Position: S23-F202). Human LIF shares 78% and 82% amino acid (aa) sequences identity with mouse and rat LIF, respectively.
Isotyp IgG
Kreuzreaktivität (Details) No cross reactivity with other proteins.
Produktmerkmale Rabbit IgG polyclonal antibody for Leukemia inhibitory factor(LIF) detection. Tested with WB, IHC-P in Human.
Gene Name: leukemia inhibitory factor
Protein Name: Leukemia inhibitory factor
Reinigung Immunogen affinity purified.
Plasmids, Primers & others Plasmide, Primers & weitere LIF products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung LIF (LIF Antibody Abstract)
Hintergrund LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.

Synonyms: CDF antibody|Cholinergic Differentiation Factor antibody|D factor antibody|DIA antibody|Differentiation inducing factor antibody|differentiation inhibitory activity antibody|Differentiation stimulating factor antibody|Differentiation-stimulating factor antibody|Emfilermin antibody|Hepatocyte stimulating factor III antibody|HILDA antibody|Human interleukin in DA cells antibody|Leukemia inhibitory factor antibody|LIF antibody|LIF_HUMAN antibody|Melanoma derived LPL inhibitor antibody|Melanoma-derived LPL inhibitor antibody|MLPLI antibody
Gen-ID 3976
UniProt P15018
Pathways JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
Applikations-hinweise WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for LIF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Kommentare

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Konzentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Leukemia Inhibitory Factor (LIF) (AA 23-202) antibody (ABIN3043403) Anti-LIF Picoband antibody, IHC(P): Human Placenta Tissue
Western Blotting (WB) image for anti-Leukemia Inhibitory Factor (LIF) (AA 23-202) antibody (ABIN3043403) Anti-LIF Picoband antibody, All lanes: Anti-LIF at 0.5ug/ml WB: Recombinant Human LI...
Immunohistochemistry (IHC) image for anti-Leukemia Inhibitory Factor (LIF) (AA 23-202) antibody (ABIN3043403) Anti-LIF Picoband antibody, IHC(P): Human Lung Cancer Tissue
Produkt verwendet in: Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012 (PubMed).

Xu, Sun, Li, Wu, Zhang, Feng: "Pinopodes, leukemia inhibitory factor, integrin-?3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss." in: Fertility and sterility, Vol. 98, Issue 2, pp. 389-95, 2012 (PubMed).

Li, Xu, Chen, Sun: "Effects of hydrosalpinx on pinopodes, leukaemia inhibitory factor, integrin beta3 and MUC1 expression in the peri-implantation endometrium." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 151, Issue 2, pp. 171-5, 2010 (PubMed).

Pei, Yu, Shi, Jia: "The effects of mifepristone on the expressions of osteopontin, interleukin-6 and leukemia inhibitory factor in the villi of early pregnant women." in: Contraception, Vol. 82, Issue 4, pp. 379-84, 2010 (PubMed).

Hu, Feng, Tang, Zhu, Lin, Yu: "LIF upregulates expression of NK-1R in NHBE cells." in: Mediators of inflammation, Vol. 2006, Issue 5, pp. 84829, 2007 (PubMed).

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