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PKC eta Antikörper (AA 30-389)

Der Kaninchen Polyklonal Anti-PKC eta-Antikörper wurde für WB, IHC, ICC und FACS validiert. Er ist geeignet, PKC eta in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN3043361

Kurzübersicht für PKC eta Antikörper (AA 30-389) (ABIN3043361)

Target

Alle PKC eta (PRKCH) Antikörper anzeigen
PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Reaktivität

  • 57
  • 28
  • 19
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Human

Wirt

  • 63
  • 5
Kaninchen

Klonalität

  • 62
  • 5
Polyklonal

Konjugat

  • 47
  • 7
  • 6
  • 4
  • 2
  • 2
Dieser PKC eta Antikörper ist unkonjugiert

Applikation

  • 58
  • 37
  • 28
  • 20
  • 7
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 10
    • 10
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 30-389

    Verwendungszweck

    Anti-PKC eta/PRKCH Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-PKC eta/PRKCH Antibody Picoband® (ABIN3043361). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry, 0.5-1 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: PRKCH protein kinase C, eta". 2. Bacher N, Zisman Y, Berent E, Livneh E (Apr 1992). "Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart". Mol Cell Biol 12 (3): 1404.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

  • Target

    PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

    Andere Bezeichnung

    PRKCH

    Hintergrund

    Synonyms: Protein kinase C eta type,2.7.11.13,PKC-L,nPKC-eta,PRKCH,PKCL, PRKCL,

    Tissue Specificity: Most abundant in lung, less in heart and skin. .

    Background: PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

    Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

    Molekulargewicht

    78 kDa

    Gen-ID

    5583

    UniProt

    P24723

    Pathways

    Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling
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