CA3 Antikörper (N-Term)

Produktnummer ABIN3043222

Produktdetails anti-CA3 Antikörper

Target: CA3 (Carbonic Anhydrase III (CA3))

Bindungsspezifität: AA 2-20, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: AKEWGYASHN GPDHWHELF

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: carbonic anhydrase III, muscle specific
Protein Name: Carbonic anhydrase 3

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CA3 (2-20aa AKEWGYASHNGPDHWHELF), different from the related mouse and rat sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CA3

Target: CA3 (Carbonic Anhydrase III (CA3))

Andere Bezeichnung: CA3 (CA3 Produkte)

Synonyme: CAIII antikoerper, Car3 antikoerper, BB219044 antikoerper, Ca3 antikoerper, Car-3 antikoerper, carbonic anhydrase 3 antikoerper, CA3 antikoerper, CAH3 antikoerper, Car3 antikoerper, Ca3 antikoerper

Hintergrund: Carbonic anhydrase III(CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family(at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. CA3 is found in high concentration in muscle. CA3 is used in conjunction with creatine kinase detection of the carrier state for Duchenne muscular dystrophy.

Synonyms: CA III antibody|CA-III antibody|Ca3 antibody|CAH3_HUMAN antibody|CAIII antibody|Car3 antibody|Carbonate dehydratase III antibody|Carbonic anhydrase 3 antibody|Carbonic anhydrase III antibody|Carbonic anhydrase III muscle specific antibody|EC 4.2.1.1 antibody

UniProt: P07451