Aurora Kinase B Antikörper (N-Term)

Produktnummer ABIN3043048

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Aurora Kinase B in WB. Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Aurora Kinase B Antikörper (N-Term) (ABIN3043048)

Target: Aurora Kinase B (AURKB)

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora Kinase B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Aurora Kinase B Antikörper

Bindungsspezifität: AA 72-87, N-Term

Verwendungszweck: Anti-Aurora B/AURKB Antibody Picoband®

Sequenz: QNKQPFTIDN FEIGRP

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Aurora B/AURKB Antibody (ABIN3043048). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Aurora B, different from the related rat sequence by two anino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Rat, Mouse
1. Andrews, P. D., Ovechkina, Y., Morrice, N., Wagenbach, M., Duncan, K., Wordeman, L., Swedlow, J. R. Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004. 2. Katayama, H., Ota, T., Morita, K., Terada, Y., Suzuki, F., Katoh, O., Tatsuka, M. Human AIM-1: cDNA cloning and reduced expression during endomitosis in megakaryocyte-lineage cells. Gene 224: 1-7, 1998. 3. Ramadan, K., Bruderer, R., Spiga, F. M., Popp, O., Baur, T., Gotta, M., Meyer, H. H. Cdc48/p97 promotes reformation of the nucleus by extracting the kinase Aurora B from chromatin. Nature 450: 1258-1262, 2007.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Andere Bezeichnung: AURKB

Hintergrund:

Synonyms: Aurora kinase B;2.7.11.1;Aurora 1;Aurora- and IPL1-like midbody-associated protein 1;Aurora/IPL1-related kinase 2;ARK-2;Aurora-related kinase 2;STK-1;Serine/threonine-protein kinase 12;Serine/threonine-protein kinase 5;Serine/threonine-protein kinase aurora-B;Aurkb;Aik2, Aim1, Airk2, Ark2, Stk1, Stk12, Stk5;

Tissue Specificity: Expressed in testis, intestine and spleen. All of them are tissues that contain a large number of proliferating cells. Expressed during S phase, in a cell-cycle-dependent fashion.

Background: AURKB (aurora kinase B) also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that STK12 is expressed in the S phase and persistently thereafter. Western blot analysis indicated that STK12 is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb STK12 transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. The AURKB gene is mapped on 17p13.1. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected AURKB from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A to AURKB. 

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Molekulargewicht: 38 kDa

UniProt: O70126

Pathways: T-Zell Rezeptor Signalweg, Zellzyklus, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades