KLF4 Antikörper (N-Term)

Produktnummer ABIN3042832

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KLF4 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für KLF4 Antikörper (N-Term) (ABIN3042832)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-KLF4 Antikörper

Bindungsspezifität: AA 93-108, N-Term

Verwendungszweck: Anti-Krueppel-like factor 4 KLF4 Antibody Picoband®

Sequenz: RRETEEFNDL LDLDFI

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Krueppel-like factor 4 KLF4 Antibody (ABIN3042832). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Moore, D. L., Blackmore, M. G., Hu, Y., Kaestner, K. H., Bixby, J. L., Lemmon, V. P., Goldberg, J. L. KLF family members regulate intrinsic axon regeneration ability. Science 326: 298-301, 2009. 2. Patel, S., Xi, Z. F., Seo, E. Y., McGaughey, D., Segre, J. A. Klf4 and corticosteroids activate an overlapping set of transcriptional targets to accelerate in utero epidermal barrier acquisition. Proc. Nat. Acad. Sci. 103: 18668-18673, 2006. 3. Wang, H., Yang, L., Jamaluddin, M. S., Boyd, D. D. The Kruppel-like KLF4 transcription factor, a novel regulator of urokinase receptor expression, drives synthesis of this binding site in colonic crypt luminal surface epithelial cells. J. Biol. Chem. 279: 22674-22683, 2004.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-KLF4 Antikörper (ABIN3042832)

Ai, Shao, Shi, Yu, Wu, Du, Zhang, Guo: "Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901." in: Stem cells international, Vol. 2016, pp. 1792573, (2016) (PubMed).

Ai, Shao, Wu, Yu, Du, Shi, Shi, Zhang, Guo: "CHIR99021 enhances Klf4 Expression through β-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150936, (2016) (PubMed).

Details zu KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Andere Bezeichnung: KLF4

Hintergrund:

Synonyms: Krueppel-like factor 4,Epithelial zinc finger protein EZF,Gut-enriched krueppel-like factor,KLF4,EZF, GKLF,

Background: Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Sequence Similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.

Molekulargewicht: 47 kDa

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance