FGF2 Antikörper (AA 143-288)

Produktnummer ABIN3042812

Der Kaninchen Polyklonal Anti-FGF2-Antikörper wurde für WB, ELISA, ICC, FACS und IF validiert. Er ist geeignet, FGF2 in Proben von Human zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für FGF2 Antikörper (AA 143-288) (ABIN3042812)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-FGF2 Antikörper

Bindungsspezifität: AA 143-288

Verwendungszweck: Anti-Fibroblast growth factor 2 FGF2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Fibroblast growth factor 2 FGF2 Antibody (ABIN3042812). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human FGF2 recombinant protein (Position: P143-S288).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Doniach, T. : Basic FGF as an inducer of anteroposterior neural pattern. Cell 83: 1067-1070, 1995. 2. Eckenstein, F. P. : Fibroblast growth factors in the nervous system. J. Neurobiol. 25: 1467-1480, 1994.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-FGF2 Antikörper (ABIN3042812)

Xiong, Sun, Liu, Xu, He et al.: "Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein ..." in: Oncology letters, Vol. 11, Issue 1, pp. 491-499, (2016) (PubMed).

Wang, Wang, Yao, Wu, Tang, Gu, Li: "The fibroblast growth factor-2 arrests Mycobacterium avium sp. paratuberculosis growth and immunomodulates host response in macrophages." in: Tuberculosis (Edinburgh, Scotland), Vol. 95, Issue 4, pp. 505-14, (2015) (PubMed).

Hu, Zhang, Ren, Deng, Cai, Lei, Ping: "Effect of vacuum-assisted closure combined with open bone grafting to promote rabbit bone graft vascularization." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 1200-6, (2015) (PubMed).

Yu, Zhang, Jiang, Wang, Zhang: "Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 6718-26, (2015) (PubMed).

Ai, Liu, Sun: "Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured Müller cells." in: Molecular medicine reports, Vol. 7, Issue 1, pp. 16-20, (2014) (PubMed).

Zhou, Ma, Si, Li, Xu, Tu, Wang: "MicroRNA-503 targets FGF2 and VEGFA and inhibits tumor angiogenesis and growth." in: Cancer letters, Vol. 333, Issue 2, pp. 159-69, (2013) (PubMed).

Details zu FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: FGF2

Hintergrund:

Synonyms: Fibroblast growth factor 2,FGF-2,Basic fibroblast growth factor,bFGF,Heparin-binding growth factor 2,HBGF-2,FGF2,FGFB,

Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .

Background: FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96 % and 97 % amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Sequence Similarities: Belongs to the heparin-binding growth factors family.

Molekulargewicht: 17-21 kDa

UniProt: P09038

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine