Cathepsin B Antikörper (AA 80-333)

Produktnummer ABIN3042773

Der Kaninchen Polyklonal Anti-Cathepsin B-Antikörper wurde für WB validiert. Er ist geeignet, Cathepsin B in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Cathepsin B Antikörper (AA 80-333) (ABIN3042773)

Target: Cathepsin B (CTSB)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cathepsin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Cathepsin B Antikörper

Bindungsspezifität: AA 80-333

Verwendungszweck: Anti-Cathepsin B/CTSB Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cathepsin B/CTSB Antibody Picoband® (ABIN3042773). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cathepsin B recombinant protein (Position: L80-D333). Human Cathepsin B shares 83% and 84% amino acid (aa) sequences identity with mouse and rat Cathepsin B, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. Chan SJ, San Segundo B, McCormick MB, Steiner DF (October 1986). "Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs". Proc. Natl. Acad. Sci. U.S.A. 83 (20): 7721-5. 2. Cao L, Taggart RT, Berquin IM, Moin K, Fong D, Sloane BF (February 1994). "Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene". Gene 139 (2): 163-9. 3. Wang, X., Chan, S. J., Eddy, R. L., Byers, M. G., Fukushima, Y., Henry, W. M., Haley, L. L., Steiner, D. F., Shows, T. B. : Chromosome assignment of cathepsin B (CTSB) to 8p22 and cathepsin H (CTSH) to 15q24-q25. (Abstract) Cytogenet. Cell Genet. 46: 710-711, 1987. 4. Mueller-Steiner S, Zhou Y, Arai H, Roberson ED, Sun B, Chen J, Wang X, Yu G, Esposito L, Mucke L, Gan L (September 2006). "Antiamyloidogenic and neuroprotective functions of cathepsin B: implications for Alzheimer's disease". Neuron 51 (6): 703-14.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Cathepsin B Antikörper (ABIN3042773)

Zheng, Li, Wang, Wang, Liao, Hu, Fan, Kang, Zeng, Wu, Wu, Zhang, Wang, He et al.: "Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage ..." in: Autophagy, Vol. 12, Issue 9, pp. 1593-613, (2017) (PubMed).

Zhang, Mai, Yang, Dong, Zheng, Yang: "CTSB promotes porcine preadipocytes differentiation by degrading fibronectin and attenuating the Wnt/β-catenin signaling pathway." in: Molecular and cellular biochemistry, Vol. 395, Issue 1-2, pp. 53-64, (2014) (PubMed).

Yang, Lü, Hong, Xi, Zhang: "The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function." in: Biomaterials, Vol. 35, Issue 24, pp. 6195-205, (2014) (PubMed).

Details zu Cathepsin B

Target: Cathepsin B (CTSB)

Andere Bezeichnung: CTSB

Hintergrund:

Synonyms: Cathepsin B,3.4.22.1,APP secretase,APPS,Cathepsin B1,Cathepsin B light chain,Cathepsin B heavy chain,CTSB,CPSB,

Tissue Specificity: Expressed in all cell types so far analyzed.

Background: < a href="https://www.bosterbio.com/anti-cathepsin-b-picoband-trade-antibody-pb9046-boster.html" style="color:#ea8d28">Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. And this gene is mapped to chromosome 8p22. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Sequence Similarities: Belongs to the peptidase C1 family.

Molekulargewicht: 33 kDa

Gen-ID: 1508

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades