HSD17B6 Antikörper (C-Term)

Produktnummer ABIN3042725

Der Kaninchen Polyklonal Anti-HSD17B6-Antikörper wurde für WB und IHC validiert. Er ist geeignet, HSD17B6 in Proben von Human zu detektieren.

Kurzübersicht für HSD17B6 Antikörper (C-Term) (ABIN3042725)

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSD17B6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-HSD17B6 Antikörper

Bindungsspezifität: AA 300-317, C-Term

Verwendungszweck: Anti-HSD17B6 Antibody Picoband®

Sequenz: SLADYILTRS WPKPAQAV

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-HSD17B6 Antibody (ABIN3042725). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Baker ME (Feb 2001). "Evolution of 17beta-hydroxysteroid dehydrogenases and their role in androgen, estrogen and retinoid action". Mol Cell Endocrinol 171 (1-2): 211-5. doi:10.1016/S0303-7207(00)00414-7. PMID 11165032. 2. Biswas, M. G., Russell, D. W. Expression cloning and characterization of oxidative 17-beta- and 3-alpha -hydroxysteroid dehydrogenases from rat and human prostate. J. Biol. Chem. 272: 15959-15966, 1997.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu HSD17B6

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Andere Bezeichnung: HSD17B6

Hintergrund:

Synonyms: 17-beta-hydroxysteroid dehydrogenase type 6,17-beta-HSD 6,17-beta-HSD6,1.1.1.105,1.1.1.239,1.1.1.62,3-alpha->beta-hydroxysteroid epimerase,3-alpha->beta-HSE,Oxidative 3-alpha hydroxysteroid dehydrogenase,Short chain dehydrogenase/reductase family 9C member 6,HSD17B6,RODH, SDR9C6,

Tissue Specificity: Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain. .

Background: Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action, 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.

Sequence Similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Molekulargewicht: 35 kDa

UniProt: O14756

Pathways: Steroid Hormone Biosynthesis