EPH Receptor B3 Antikörper (C-Term)

Produktnummer ABIN3042663

Dieser Anti-EPH Receptor B3 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von EPH Receptor B3 in WB und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für EPH Receptor B3 Antikörper (C-Term) (ABIN3042663)

Target: EPH Receptor B3 (EPHB3)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPH Receptor B3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-EPH Receptor B3 Antikörper

Bindungsspezifität: AA 982-998, C-Term

Verwendungszweck: Anti-Eph receptor B3/EPHB3 Antibody Picoband®

Sequenz: SIQDMRLQMN QTLPVQV

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Eph receptor B3/EPHB3 Antibody (ABIN3042663). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor B3, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Bohme, B., Holtrich, U., Wolf, G., Luzius, H., Grzeschik, K.-H., Strebhardt, K., Rubsamen-Waigmann, H.PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2.Oncogene 8: 2857-2862, 1993. 2. Bohme, B., VandenBos, T., Cerretti, D. P., Park, L. S., Holtrich, U., Rubsamen-Waigmann, H., Strebhardt, K.Cell-cell adhesion mediated by binding of membrane-anchored ligand LERK-2 to the EPH-related receptor human embryonal kinase 2 promotes tyrosine kinase activity.J. Biol. Chem. 271: 24747-24752, 1996.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Andere Bezeichnung: EPHB3

Hintergrund:

Synonyms: Ephrin type-B receptor 3,2.7.10.1,EPH-like tyrosine kinase 2,EPH-like kinase 2,Embryonic kinase 2,EK2,hEK2,Tyrosine-protein kinase TYRO6,EPHB3,ETK2, HEK2, TYRO6,

Tissue Specificity: Ubiquitous.

Background: Ephrin Receptor EphB3, is also known as human embryo kinase2 (HEK2) or Eph-like tyrosine kinase2 (ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases (LERKs), namely, LERK2 (EFNB1) and LERK5 (EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Molekulargewicht: 130 kDa

UniProt: P54753

Pathways: RTK Signalweg