COX1 Antikörper (N-Term)

Produktnummer ABIN3042632

Produktdetails anti-COX1 Antikörper

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Bindungsspezifität: AA 2-14, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-MTCO1/MT-CO1 Antibody Picoband®

Sequenz: FADRWLFSTN HKD

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-MTCO1/MT-CO1 Antibody (ABIN3042632). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. Kadenbach, B., Jarausch, J., Hartmann, R., Merle, P. : Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure. Anal. Biochem. 129: 517-521, 1983. 2. Shoffner, J. M., Wallace, D. C. : Oxidative phosphorylation diseases.In: Scriver, C. R., Beaudet, A. L., Sly, W. S., Valle, D. (eds.) : The Metabolic and Molecular Bases of Inherited Disease. Vol. 1. New York: McGraw-Hill (7th ed.) 1995. Pp. 1535-1609. 3. Hosler, J. P., Ferguson-Miller, S., Calhoun, M. W., Thomas, J. W., Hill, J., Lemieux, L., Ma, J., Georgiou, C., Fetter, J., Shapleigh, J., Tecklenburg, M. M. J., Babcock, G. T., Gennis, R. B. : Insight into the active-site structure and function of cytochrome oxidase by analysis of site-ed mutants of bacterial cytochrome aa3 and cytochrome bo. J. Bioenerg. Biomembr. 25: 121-136, 1993. 4. Acin-Perez, R., Bayona-Bafaluy, M. P., Bueno, M., Machicado, C., Fernandez-Silva, P., Perez-Martos, A., Montoya, J., Lopez-Perez, M. J., Sancho, J., Enriquez, J. A. : An intragenic suppressor in the cytochrome c oxidase I gene of mouse mitochondrial DNA. Hum. Molec. Genet. 12: 329-339, 2003.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu COX1

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Andere Bezeichnung: MT-CO1 (COX1 Produkte)

Hintergrund:

Synonyms: Cytochrome c oxidase subunit 1,1.9.3.1,Cytochrome c oxidase polypeptide I,MT-CO1,COI, COXI, MTCO1,

Tissue Specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Background: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Sequence Similarities: Belongs to the SNAP-25 family.

Molekulargewicht: 37 kDa

UniProt: P00395

Pathways: Proton Transport