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COX1 Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-COX1-Antikörper wurde für WB validiert. Er ist geeignet, COX1 in Proben von Human zu detektieren.
Produktnummer ABIN3042632

Kurzübersicht für COX1 Antikörper (N-Term) (ABIN3042632)

Target

Alle COX1 Antikörper anzeigen
COX1 (Cytochrome C Oxidase Subunit I (COX1))

Reaktivität

  • 42
  • 31
  • 20
  • 3
Human

Wirt

  • 60
  • 4
  • 2
Kaninchen

Klonalität

  • 62
  • 4
Polyklonal

Konjugat

  • 27
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser COX1 Antikörper ist unkonjugiert

Applikation

  • 43
  • 25
  • 13
  • 13
  • 13
  • 12
  • 9
  • 6
  • 4
  • 3
  • 2
Western Blotting (WB)
  • Bindungsspezifität

    • 15
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-14, N-Term

    Verwendungszweck

    Anti-MTCO1/MT-CO1 Antibody Picoband®

    Sequenz

    FADRWLFSTN HKD

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-MTCO1/MT-CO1 Antibody (ABIN3042632). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human
    1. Kadenbach, B., Jarausch, J., Hartmann, R., Merle, P. : Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure. Anal. Biochem. 129: 517-521, 1983. 2. Shoffner, J. M., Wallace, D. C. : Oxidative phosphorylation diseases.In: Scriver, C. R., Beaudet, A. L., Sly, W. S., Valle, D. (eds.) : The Metabolic and Molecular Bases of Inherited Disease. Vol. 1. New York: McGraw-Hill (7th ed.) 1995. Pp. 1535-1609. 3. Hosler, J. P., Ferguson-Miller, S., Calhoun, M. W., Thomas, J. W., Hill, J., Lemieux, L., Ma, J., Georgiou, C., Fetter, J., Shapleigh, J., Tecklenburg, M. M. J., Babcock, G. T., Gennis, R. B. : Insight into the active-site structure and function of cytochrome oxidase by analysis of site-ed mutants of bacterial cytochrome aa3 and cytochrome bo. J. Bioenerg. Biomembr. 25: 121-136, 1993. 4. Acin-Perez, R., Bayona-Bafaluy, M. P., Bueno, M., Machicado, C., Fernandez-Silva, P., Perez-Martos, A., Montoya, J., Lopez-Perez, M. J., Sancho, J., Enriquez, J. A. : An intragenic suppressor in the cytochrome c oxidase I gene of mouse mitochondrial DNA. Hum. Molec. Genet. 12: 329-339, 2003.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    COX1 (Cytochrome C Oxidase Subunit I (COX1))

    Andere Bezeichnung

    MT-CO1

    Hintergrund

    Synonyms: Cytochrome c oxidase subunit 1,1.9.3.1,Cytochrome c oxidase polypeptide I,MT-CO1,COI, COXI, MTCO1,

    Tissue Specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

    Background: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

    Sequence Similarities: Belongs to the SNAP-25 family.

    Molekulargewicht

    37 kDa

    UniProt

    P00395

    Pathways

    Proton Transport
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