GM-CSF Antikörper (AA 18-141)

Produktnummer ABIN3042527

Produktdetails anti-GM-CSF Antikörper

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Bindungsspezifität: AA 18-141

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GM-CSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-GM-CSF/CSF2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-GM-CSF/CSF2 Antibody Picoband® (ABIN3042527). Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Western blot, 0.1-0.5 μg/mL, Mouse
1. Le Beau, M. M., Espinosa, R., III, Neuman, W. L., Stock, W., Roulston, D., Larson, R. A., Keinanen, M., Westbrook, C. A.Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.Proc. Nat. Acad. Sci. 90: 5484-5488, 1993. 2. Park, B. K., Zhang, H., Zeng, Q., Dai, J., Keller, E. T., Giordano, T., Gu, K., Shah, V., Pei, L., Zarbo, R. J., McCauley, L., Shi, S., Chen, S., Wang, C.-Y.NF-kappa-B in breast cancer cells promotes osteolytic bone metastasis by inducing osteoclastogenesis via GM-CSF.Nature Med. 13: 62-69, 2007. 3. Uchida, K., Beck, D. C., Yamamoto, T., Berclaz, P.-Y., Abe, S., Staudt, M. K., Carey, B. C., Filippi, M.-D., Wert, S. E., Denson, L. A., Puchalski, J. T., Hauck, D. M., Trapnell, B. C.GM-CSF autoantibodies and neutrophil dysfunction in pulmonary alveolar proteinosis.New Eng. J. Med. 356: 567-579, 2007.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-GM-CSF Antikörper (ABIN3042527)

Fang, Shi, Meng, Li, Peng: "The Balance between Conventional DCs and Plasmacytoid DCs Is Pivotal for Immunological Tolerance during Pregnancy in the Mouse." in: Scientific reports, Vol. 6, pp. 26984, (2018) (PubMed).

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: CSF2 (CSF2 Produkte)

Hintergrund:

Synonyms: Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF,Csf2,Csfgm,

Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.

Background: GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

Sequence Similarities: Belongs to the GM-CSF family.

Molekulargewicht: 17 kDa

UniProt: P01587

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin