Lamin B1 Antikörper (AA 266-583)

Produktnummer ABIN3042505

Produktdetails anti-Lamin B1 Antikörper

Target: Lamin B1 (LMNB1)

Bindungsspezifität: AA 266-583

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Lamin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck: Anti-Lamin B1/LMNB1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Lamin B1/LMNB1 Antibody Picoband® (ABIN3042505). Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Lamin B1 recombinant protein (Position: Q266-C583). Human Lamin B1 shares 95.9% and 95% amino acid (aa) sequence identity with mouse and rat Lamin B1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunoprecipitation, 0.5-2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Pollard, K. M., Chan, E. K., Grant, B. J., Sullivan, K. E., Tan, E. M., Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990. 2. Tsai, M.-Y., Wang, S., Heidinger, J. M., Shumaker, D. K., Adam, S. A., Goldman, R. D., Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Lamin B1 Antikörper (ABIN3042505)

Chen, Luo, Lin, Kuang, Song: "Simulated microgravity inhibits osteogenic differentiation of mesenchymal stem cells via depolymerizing F-actin to impede TAZ nuclear translocation." in: Scientific reports, Vol. 6, pp. 30322, (2018) (PubMed).

Timoshevskiy, Herdy, Keinath, Smith: "Cellular and Molecular Features of Developmentally Programmed Genome Rearrangement in a Vertebrate (Sea Lamprey: Petromyzon marinus)." in: PLoS genetics, Vol. 12, Issue 6, pp. e1006103, (2017) (PubMed).

Shen, Sun, Xia, Meng, Lei, Zhao, Tang, Xue, Chen: "Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose." in: International journal of molecular medicine, Vol. 38, Issue 3, pp. 729-36, (2017) (PubMed).

Chen, Jiang, Gu, Lai, Liu, Zhang: "Protection of Nrf2 against arsenite-induced oxidative damage is regulated by the cyclic guanosine monophosphate-protein kinase G signaling pathway." in: Environmental toxicology, Vol. 32, Issue 8, pp. 2004-2020, (2017) (PubMed).

Zhang, Zhang, Zhang, Jiao, Zhang, Wang, Gong: "Glutamine ameliorates lipopolysaccharide-induced cardiac dysfunction by regulating the toll-like receptor 4/mitogen-activated protein kinase/nuclear factor-kB signaling pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5825-5832, (2017) (PubMed).

Tang, Wang, Chen, Xu, Ye: "Clinicopathologic and prognostic significance of p21 (Cip1/Waf1) expression in bladder cancer." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4999-5007, (2016) (PubMed).

Chen, Lai, Yang, Ruan, Chaugai, Ning, Chen, Wang: "Trimetazidine prevents macrophage-mediated septic myocardial dysfunction via activation of the histone deacetylase sirtuin 1." in: British journal of pharmacology, Vol. 173, Issue 3, pp. 545-61, (2016) (PubMed).

Bai, Zhang, Shen, Sun, Xia, Zhang, Liu, Wang, Yuan: "Hyperglycemia and hyperlipidemia blunts the Insulin-Inpp5f negative feedback loop in the diabetic heart." in: Scientific reports, Vol. 6, pp. 22068, (2016) (PubMed).

Wu, Liu, Duan, Gao, Li, Wang, Song, Shao, Yang, Yang: "The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration." in: Scientific reports, Vol. 6, pp. 30563, (2016) (PubMed).

Shen, Wang, Wang, Wang, Yang, Shen, Wang, Guo, Liu, Xia, Jia, Wang: "Activation of volume-sensitive outwardly rectifying chloride channel by ROS contributes to ER stress and cardiac contractile dysfunction: involvement of CHOP through Wnt." in: Cell death & disease, Vol. 5, pp. e1528, (2015) (PubMed).

Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, (2014) (PubMed).

Liu, Ma, Wu, Li, Zhang, Han, Wang, Reindl, Wu, Ma: "Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-?B signaling." in: BMC cancer, Vol. 14, pp. 686, (2014) (PubMed).

Gao, Wu, Liu, Li, Song, Teng, Sheng, Liu, Yao, Chen, Jiang, Chen, Huang: "Interaction of NS2 with AIMP2 facilitates the switch from ubiquitination to SUMOylation of M1 in influenza A virus-infected cells." in: Journal of virology, Vol. 89, Issue 1, pp. 300-11, (2014) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Zhang, Zhou, Wu, Zhang, Chen, Zha, Niu, Lu, Hao, Sun, Sun, Peng, Wang: "Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)-ginsenoside Rh2 in MCF-7/Adr cells." in: British journal of pharmacology, Vol. 165, Issue 1, pp. 120-34, (2012) (PubMed).

Details zu Lamin B1

Target: Lamin B1 (LMNB1)

Andere Bezeichnung: LMNB1 (LMNB1 Produkte)

Hintergrund:

Synonyms: Lamin-B1,LMNB1,LMN2, LMNB,

Tissue Specificity: Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. .

Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Sequence Similarities: Belongs to the intermediate filament family.

Molekulargewicht: 67 kDa

Gen-ID: 4001

UniProt: P20700

Pathways: Apoptose, Caspase Kaskade in der Apoptose